Protein Arrays pp 123-134 | Cite as

Evaluation of Antibodies and Microarray Coatings As a Prerequisite for the Generation of Optimized Antibody Microarrays

  • Philipp Angenendt
  • Jörn Glökler
Part of the Methods in Molecular Biology book series (MIMB, volume 264)

Abstract

Antibody microarrays are becoming a major tool for the parallel analysis of complex samples. So far, many efforts have been made to increase the complexity and sensitivity of antibody microarrays. In contrast to enzyme-linked immunosorbent assay (ELISA) experiments, not all antibodies remain functional in the microarray format. Sensitivity is very much dependent on the type of coating and its application. The method described in this chapter is a quick and reliable method that has been very useful in determining the functionality of antibodies and the suitability of coatings for antibody microarrays. At the same time, a detailed description of how to prepare an inexpensive and highly efficient antibody microarray surface is given.

Key Words

Antibody microarray functionality poly-L-lysine surface coating ELISA 

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Philipp Angenendt
    • 1
  • Jörn Glökler
    • 1
  1. 1.Protein Expression Group, Department LehrachMax Planck Institute for Molecular GeneticsBerlinGermany

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