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The Application of LTR Retrotransposons as Molecular Markers in Plants

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Book cover Mobile Genetic Elements

Part of the book series: Methods in Molecular Biology ((MIMB,volume 260))

Abstract

Retrotransposons are major, dispersed components of most eukaryotic genomes. They replicate by a cycle of transcription, reverse transcription, and integration of new copies, without excising from the genome in the process. Because they represent a major share of the genome, cause easily detectable genetic changes having known ancestral and derived states, and contain conserved regions for which polymerase chain reaction (PCR) primers may be designed, retrotransposon insertions can be exploited as powerful molecular marker systems. Here, we describe the background and strategies, as well as give detailed laboratory protocols, for four key retrotransposon-based methods: SSAP, IRAP, REMAP, and RBIP. The SSAP, IRAP, and REMAP methods are multiplex and generate anonymous marker bands; RBIP scores individual loci, much as microsatellite-based marker systems do. The methods are variously suited to marker detection on agarose and polyacrylamide slab gels, slab and capillary sequencing devices, and arrays on solid supports. The different strengths and weaknesses of these approaches and their performance relative to conventional marker methods are discussed, together with their applicability to marker-assisted breeding, phylogenetic analyses, biodiversity determinations, and evolutionary studies.

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Author information

Authors and Affiliations

  1. MTT/BI Plant Genomics Laboratory, Institute of Biotechnology, University of Helsinki, Helsinki, Finland

    Alan H. Schulman

  2. Plant Research Unit, The University of Dundee at SCRI, Dundee, Scotland, UK

    Andrew J. Flavell

  3. Department of Crop Genetics, The John Innes Centre, Colney Lane, Norwich, UK

    T. H. Noel Ellis

Authors
  1. Alan H. Schulman
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  2. Andrew J. Flavell
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  3. T. H. Noel Ellis
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Editor information

Editors and Affiliations

  1. AG General Genetics, Institute of Medical Biology, University of Vienna, Vienna, Austria

    Wolfgang J. Miller

  2. Laboratoire Populations, Génétique et Evolution, CNRS, Gif-sur-Yvette, France

    Pierre Capy

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© 2004 Humana Press Inc., Totowa, NJ

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Schulman, A.H., Flavell, A.J., Ellis, T.H.N. (2004). The Application of LTR Retrotransposons as Molecular Markers in Plants. In: Miller, W.J., Capy, P. (eds) Mobile Genetic Elements. Methods in Molecular Biology, vol 260. Humana Press. https://doi.org/10.1385/1-59259-755-6:145

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  • DOI: https://doi.org/10.1385/1-59259-755-6:145

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-007-6

  • Online ISBN: 978-1-59259-755-0

  • eBook Packages: Springer Protocols

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