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Identification and Quantitation of G-Protein α-Subunits

  • Ian Mullaney
  • Graeme Milligan
Part of the Methods in Molecular Biology book series (MIMB, volume 259)

Abstract

The demonstration that many intracellular signaling processes are mediated by a family of closely related guanine nucleotide binding proteins (G-proteins) has led to the development of specific techniques that can be used to identify which of these polypeptide(s) is involved on receptor activation by ligand. In addition, these methods can be used to probe the specificity of the interaction and to yield information about the stoichiometries involved.

Key Words

G-protein immunoblotting pertussis toxin 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Ian Mullaney
    • 1
  • Graeme Milligan
    • 1
  1. 1.Molecular Pharmacology GroupUniversity of GlasgowGlasgowScotland, UK

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