Abstract
Many functional studies require the transfection of bacterial artificial chromosome (BAC) clones into mammalian cells. Because most BAC vectors do not have a mammalian selection marker or do not have one suitable for all cell types, it is usually necessary to modify BAC clones to include a selection marker, a “retrofitting” process. Different BAC retrofitting strategies have been developed over the last 5 yr and are classified in the reference section (1–11).
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References
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© 2004 Humana Press Inc., Totowa, NJ
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Wang, Z., Longacre, A., Engler, P. (2004). Retrofitting BACs With a Selectable Marker for Transfection. In: Zhao, S., Stodolsky, M. (eds) Bacterial Artificial Chromosomes. Methods in Molecular Biology, vol 256. Humana Press. https://doi.org/10.1385/1-59259-753-X:069
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DOI: https://doi.org/10.1385/1-59259-753-X:069
Publisher Name: Humana Press
Print ISBN: 978-0-89603-989-6
Online ISBN: 978-1-59259-753-6
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