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Retrofitting BACs With a Selectable Marker for Transfection

  • Zunde Wang
  • Angelika Longacre
  • Peter Engler
Part of the Methods in Molecular Biology book series (MIMB, volume 256)

Abstract

Many functional studies require the transfection of bacterial artificial chromosome (BAC) clones into mammalian cells. Because most BAC vectors do not have a mammalian selection marker or do not have one suitable for all cell types, it is usually necessary to modify BAC clones to include a selection marker, a “retrofitting” process. Different BAC retrofitting strategies have been developed over the last 5 yr and are classified in the reference section (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11).

Keywords

Embryonic Stem Cell Bacterial Artificial Chromosome Bacterial Artificial Chromosome Clone loxP Site Vector Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Zunde Wang
    • 1
  • Angelika Longacre
    • 2
  • Peter Engler
    • 2
  1. 1.Genomics DivisionEpiGenX PharmaceuticalsSanta Barbara
  2. 2.Department of Molecular Genetics and Cell BiologyUniversity of ChicagoChicago

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