Abstract
]The ultimate goal of the Human Genome Project is to establish the DNA sequence of human and model organism genomes as the critical first step in understanding disease, development and evolution. To accomplish this goal and a broad spectrum of applications requires integration of genome sequence information to genetic markers (expressed sequence tag/cDNA/gene transcripts content) and to reagents that can be seen through a microscope and linked to cytogenetic landmarks. Such linkage/integration should be dense large fragments and for reagents well characterized with respect to low-copy repeats that are present at multiple other points in the genome. Therefore, ideally, the same templates should be used as an integrating framework of entry points for sequencing and then applied to gene isolation and mapping, studies of genome organization and evolution, and a myriad of clinical applications (1).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Korenberg, J. R., Chen, X. N., Sun, Z., et al. (1999) Human genome anatomy: BACs integrating the genetic and cytogenetic maps for bridging genome and bio-medicine. Genome Res. 9, 994–1001.
Nath, J. and Johnson, K. L. (2000) A review of fluorescence in situ hybridization (FISH): current status and future prospects. Biotech. Histochem. 75, 54–78.
Korenberg, J. R. and Chen, X. N. (1995) Human cDNA mapping using a high-resolution R-banding technique and fluorescence in situ hybridization. Cytogenet. Cell Genet. 69, 196–200.
Johnson, G. D. and Nogueira Araujo, G. M. (1981) A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods 43, 349–350.
Mitelman, E. (1994) ISCN 1995 An International System for Human Cytogenetic Nomenclature. Chap. 2.2.2 p. 7, Fig. 1.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Chen, XN., Korenberg, J.R. (2004). BAC Mapping Using Fluorescence In Situ Hybridization. In: Zhao, S., Stodolsky, M. (eds) Bacterial Artificial Chromosomes. Methods in Molecular Biology™, vol 255. Humana Press. https://doi.org/10.1385/1-59259-752-1:131
Download citation
DOI: https://doi.org/10.1385/1-59259-752-1:131
Publisher Name: Humana Press
Print ISBN: 978-0-89603-988-9
Online ISBN: 978-1-59259-752-9
eBook Packages: Springer Protocols