Abstract
Studying large genomic regions at the molecular level requires access to the DNA representing such sites. The availability of large and stable clones derived from target genomic regions is essential for detailed analysis such as sequencing. Since its introduction in 1992, the bacterial artificial chromosome (BAC) library system has been widely employed as a standard cloning system for mapping and sequencing the genomes of human and model organisms (1), as well as in a variety of other research areas where large DNA insert-containing clones are needed (2–24). With the end of the Human Genome Project drawing near (25), the genome-sequencing community is now directing its efforts at sequencing commercially important organisms. BAC library construction and high-throughput screening has become an indispensable tool for the isolation of large chromosomal DNA fragments necessary for such projects.
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References
Shizuya, H., Birren, B., Kim, U. J., Mancino, V., Slepak, T, Tachiiri, Y., and Simon, M. (1992) Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89, 8794–8797.
Woo, S. S., Jiang, J., Gill, B. S., Paterson, A. H., and Wing, R. A. (1994) Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor. Nucleic Acids Res. 22, 4922–4931.
Wang, M., Chen, X. N, Shouse, S., et al. (1994) Construction and characterization of a human chromosome 2-specific BAC library. Genomics 24, 527–534.
Wang, G. L., Holsten, T. E., Song, W. Y., Wang, H. P., and Ronald, P. C. (1995) Construction of a rice bacterial artificial chromosome library and identification of clones linked to the Xa-21 disease resistance locus. Plant J. 7, 525–533.
Cai, L., Taylor, J. F., Wing, R. A., Gallagher, D. S., Woo, S. S., and Davis, S. K. (1995) Construction and characterization of a bovine bacterial artificial chromosome library. Genomics 29, 413–425.
Kim, U. J., Shizuya, H., Chen, X. N., Deaven, L., Speicher, S., Solomon, J., Korenberg, J., and Simon, M. I. (1995) Characterization of a human chromosome 22 enriched bacterial artificial chromosome sublibrary. Genet. Anal. 12, 73–79.
Stone, N. E., Fan, J. B., Willour, V., Pennacchio, L. A., Warrington, J. A., Hu, A., de la Chapelle, A., Lehesjoki, A. E., Cox, D. R., and Myers, R. M. (1996) Construction of a 750-kb bacterial clone contig and restriction map in the region of human chromosome 21 containing the progressive myoclonus epilepsy gene. Genome Res. 6, 218–225.
Schmitt, H., Kim, U. J., Slepak, T., Blin, N., Simon, M. I., and Shizuya, H. (1996) Framework for a physical map of the human 22q13 region using bacterial artificial chromosomes (BACs). Genomics 33, 9–20.
Diaz-Perez, S. V., Crouch, V. W., and Orbach, M. J. (1996) Construction and characterization of a Magnaporthe grisea bacterial artificial chromosome library. Fungal Genet. Biol. 20, 280–288.
Hubert, R. S., Mitchell, S., Chen, X. N., et al. (1997) BAC and PAC contigs covering 3.5 Mb of the Down syndrome congenital heart disease region between D21S55 and MX1 on chromosome 21. Genomics 41, 218–226.
Zimmer, R. and Verrinder Gibbins, A. M. (1997) Construction and characterization of a large-fragment chicken bacterial artificial chromosome library. Genomics 42, 217–226.
Schibler, L., Vaiman, D., Oustry, A., Guinec, N., Dangy-Caye, A. L., Billault, A., and Cribiu, E. P. (1998) Construction and extensive characterization of a goat bacterial artificial chromosome library with threefold genome coverage. Mamm. Genome 9, 119–124.
Mozo, T., Fischer, S., Shizuya, H., and Altmann, T. (1998) Construction and characterization of the IGF Arabidopsis BAC library. Mol. Gen. Genet. 258, 562–570.
Kouprina, N., Campbell, M., Graves, J., Campbell, E., Meincke, L., Tesmer, J., Grady, D. L., Doggett, N. A., Moyzis, R. K., Deaven, L. L., and Larionov, V. (1998) Construction of human chromosome 16-and 5-specific circular YAC/BAC libraries by in vivo recombination in yeast (TAR cloning). Genomics 53, 21–28.
Li, R., Mignot, E., Faraco, J., Kadotani, H., Cantanese, J., Zhao, B., Lin, X., Hinton, L., Ostrander, E. A., Patterson, D. F, and de Jong, P. J. (1999) Construction and characterization of an eightfold redundant dog genomic bacterial artificial chromosome library. Genomics 58, 9–17.
Vaiman, D., Billault, A., Tabet-Aoul, K., Schibler, L., Vilette, D., Oustry-Vaiman, A., Soravito, C., and Cribiu, E. P. (1999) Construction and characterization of a sheep BAC library of three genome equivalents. Mamm. Genome 10, 585–587.
Wu, C., Asakawa, S., Shimizu, N., Kawasaki, S., and Yasukochi, Y. (1999) Construction and characterization of bacterial artificial chromosome libraries from the silkworm, Bombyx mori. Mol. Gen. Genet. 261, 698–706.
Capela, D., Barloy-Hubler, F., Gatius, M. T., Gouzy, J., and Galibert, F. (1999) A high-density physical map of Sinorhizobium meliloti 1021 chromosome derived from bacterial artificial chromosome library. Proc. Natl. Acad. Sci. USA 96, 9357–9362.
Suzuki, K., Asakawa, S., Iida, M., Shimanuki, S., Fujishima, N., Hiraiwa, H., Murakami, Y., Shimizu, N., and Yasue, H. (2000) Construction and evaluation of a porcine bacterial artificial chromosome library. Anim. Genet. 31, 8–12.
Song, J., Dong, F., and Jiang, J. (2000) Construction of a bacterial artificial chromosome (BAC) library for potato molecular cytogenetics research. Genome 43, 199–204.
Han, C. S., Sutherland, R. D., Jewett, P. B., et al. (2000) Construction of a BAC contig map of chromosome 16q by two-dimensional overgo hybridization. Genome Res. 10, 714–721.
Fu, H. and Dooner, H. K. (2000) A gene-enriched BAC library for cloning large allele-specific fragments from maize: isolation of a 240-kb contig of the bronze region. Genome Res. 10, 866–873.
Buitkamp, J., Kollers, S., Durstewitz, G., Fries, R., Welzel, K., Schafer, K., Keller-mann, A., and Lehrach, H. (2000) Construction and characterization of a gridded cattle BAC library. Anim. Genet. 31, 347–351.
Rogel-Gaillard, C., Piumi, F., Billault, A., Bourgeaux, N., Save, J. C., Urien, C., Salmon, J., and Chardon, P. (2000) Construction of a rabbit bacterial artificial chromosome (BAC) library: application to the mapping of the major histocompatibility complex to position 12q.1.1. Mamm. Genome 12, 253–255.
Venter, J. C,, Adams, M. D., Myers, E. W., et al. (2001) The sequence of the human genome. Science 291, 1304–1351.
Kim, U.-J., Birren, B. W., Slepak, T., Mancino, V., Boysen, C., Kang, H. L., Simon, M. I., and Shizuya, H. (1996) Construction and characterization of a human bacterial artificial chromosome library. Genomics 34, 213–218.
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Lim, CS., Kim, UJ. (2004). Hybridization-Based Selection of BAC Clones. In: Zhao, S., Stodolsky, M. (eds) Bacterial Artificial Chromosomes. Methods in Molecular Biology™, vol 255. Humana Press. https://doi.org/10.1385/1-59259-752-1:101
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DOI: https://doi.org/10.1385/1-59259-752-1:101
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