Abstract
The evolutionarily conserved DExD/H-box proteins are essential for all RNA-related biological processes. They are thought to modulate the structure and function of specific RNAs and/or ribonucleoprotein particles by using their intrinsic RNA-dependent ATPase activities to achieve the desired conformational changes. A number of DExD/H-box proteins have been shown to unwind short RNA duplexes in vitro, a hallmark of the so-called RNA helicases or unwindases. However, some are unable to do so, perhaps because of requirements for cofactors. Here, we present a “solid-state” method that may allow investigators to overcome such problems.
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© 2004 Humana Press Inc., Totowa, NJ
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Tseng-Rogenski, S.S., Chang, TH. (2004). RNA Unwinding Assay for DExD/H-Box RNA Helicases. In: Schoenberg, D.R. (eds) mRNA Processing and Metabolism. Methods in Molecular Biology™, vol 257. Humana Press. https://doi.org/10.1385/1-59259-750-5:093
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DOI: https://doi.org/10.1385/1-59259-750-5:093
Publisher Name: Humana Press
Print ISBN: 978-1-58829-225-4
Online ISBN: 978-1-59259-750-5
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