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Methods for the Analysis of Adenosine-to-Inosine Editing in RNA

  • Zuo Zhang
  • Gordon G. Carmichael
Part of the Methods in Molecular Biology™ book series (MIMB, volume 257)

Abstract

In this work we describe methods for the analysis of RNAs that have been edited by the double-strand RNA-specific adenosine deaminase, ADAR. These RNAs contain inosine residues that can be detected and quantified by a variety of approaches, including base hydrolysis and thin-layer chromatography, reverse transcription polymerase chain reaction, primer extension, and inosine-specific base cleavage. The most common method for the analysis of editing will be described here. This method involves complete hydrolysis of edited RNAs to nucleo-side monophosphates, followed by separation of the products using thin-layer chromatography.

Key Words

RNA editing editing inosine adenosine deaminase deaminase dsRNA thin-layer chromatography 

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Zuo Zhang
    • 1
  • Gordon G. Carmichael
    • 2
  1. 1.Cold Spring Harbor LaboratoryCold Spring Harbor
  2. 2.Department of Genetics and Developmental BiologyUniversity of Connecticut Health CenterFarmington

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