Abstract
Nitric oxide synthases (NOS) are expressed constitutively in vascular endothelial cells (eNOS; 1) and can be induced in vascular smooth muscle following stimulation with cytokines and endotoxin (inducible (i) NOS; for review, see ref. 2). The discovery and characterization of inhibitors of NOS (3,4) led to the elucidation of the biological significance of NO synthesis within the vessel wall.
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References
Palmer, R. M. J., Ferrige, A. G., and Moncada, S. (1987) Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature, 327, 524–526.
Stuehr, D. J. and Griffith, O. W. (1992) Mammalian nitric oxide synthases. Adv. Enzymol. Rel. Areas Mol. Biol. 65, 287–346.
Palmer, R. M. J., Rees, D. D., Ashton, D. S., and Moncada, S. (1988) L-Arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation. Biochem. Biophys. Res. Commun. 153, 1251–1256.
Rees, D. D., Palmer, R. M. J., Schulz, R., Hodson, H. F., and Moncada, S. (1990). Characterisation of three inhibitors of endothelial nitric oxide synthase in vitro and in vivo. Br. J. Pharm. 101, 747–752.
Radomski, M. W., Palmer, R. M. J., and Moncada, S. (1990) Glucocorticoids inhibit the expression of an inducible, but not the constitutive, nitric oxide synthase in vascular endothelial cells. Proc. Natl. Acad. Sci. USA 87, 10,043–10,047.
Rees, D. D., Cellek. S., Palmer, R. M. J., and Moncada, S. (1990) Dexamethasone prevents the induction by endotoxin of a nitric oxide synthase and the associated effects on vascular tone: an insight into endotoxin shock. Biochem. Biophys. Res. Commun. 173, 541–547.
Knowles, R. G., Salter, M., Brooks, S. L., and Moncada, S. (1990) Antiinflammatory glucocorticoids inhibit the induction of nitric oxide synthase in the lung, liver and aorta of the rat. Biochem. Biophys. Res. Commun. 172, 1042–1048.
Krebs, H. A. and Henseleit, K. (1932) Urea formation in the animal body. Z. Physiol. Chem. 210, 33–66.
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Russell, R.J. (1998). Use of Arginine Analogs as Inhibitors of Nitric Oxide Synthase in Rat-Aortic Rings. In: Titheradge, M.A. (eds) Nitric Oxide Protocols. Methods in Molecular Biology™, vol 100. Humana Press. https://doi.org/10.1385/1-59259-749-1:243
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DOI: https://doi.org/10.1385/1-59259-749-1:243
Publisher Name: Humana Press
Print ISBN: 978-0-89603-470-9
Online ISBN: 978-1-59259-749-9
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