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Enzymology of Nitric Oxide Synthases

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Nitric Oxide Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 100))

Abstract

Biologically produced nitric oxide (NO) originates from oxygen and L-arginine in the reaction catalyzed by NO synthase (NOS) enzymes (NOS, EC 1.14.13.39), first reported in the late 1980s. It was soon discovered that these enzymes have different biochemical properties depending on the tissue from which they are isolated. This suggested that there are special regulatory mechanisms that match the supply of NO exactly to its function in each tissue where it is produced. Subsequent research has confirmed that indeed these enzymes are subject to sophisticated and complex regulation. It is indispensable to understand this regulation better, both to understand the physiology of NO, and to pick targets for new therapeutic strategies.

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Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie, Karl-Franzens-Universität Graz, Graz, Austria

    Benjamin Hemmens & Bernd Mayer

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  1. Benjamin Hemmens
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  2. Bernd Mayer
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  1. University of Sussex, Brighton, UK

    Michael A. Titheradge

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© 1998 Humana Press Inc.

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Hemmens, B., Mayer, B. (1998). Enzymology of Nitric Oxide Synthases. In: Titheradge, M.A. (eds) Nitric Oxide Protocols. Methods in Molecular Biology™, vol 100. Humana Press. https://doi.org/10.1385/1-59259-749-1:1

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  • DOI: https://doi.org/10.1385/1-59259-749-1:1

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-470-9

  • Online ISBN: 978-1-59259-749-9

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