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Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques

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Book cover HPLC of Peptides and Proteins

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 251))

Abstract

Venoms have attracted significant study in recent years as a reservoir of complex libraries of natural products possessing a wide range of biological activities. Moreover, venoms contain specific and potent molecules that may be utilized in pharmaceutical development and in the production of environmentally friendly insecticides. The compositions of venoms are typically highly complex and contain a variety of molecules including proteins, peptides, and numerous types of small molecules. This complexity requires highly sensitive techniques to allow separation of these components for study. The techniques should also be able to accommodate large variations in sample size to account for the differences in venom available from different creatures (e.g., some snakes can supply up to 500 mg of crude venom from a single milking, whereas some small insects, such as ants, supply submicrogram amounts [1]). These qualities have been found and continue to be advanced in the technique high-performance liquid chromatography (HPLC), in particular reversed-phase HPLC (RP-HPLC). This technique combined with a variety of detection methods can allow the collection of a significant amount of data from very small venom samples.

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Author information

Authors and Affiliations

  1. Xenome Ltd., Indooroopilly, Queensland, Australia

    David Wilson

  2. Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia

    Paul Alewood

Authors
  1. David Wilson
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  2. Paul Alewood
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Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia

    Marie-Isabel Aguilar

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© 2004 Humana Press Inc.

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Wilson, D., Alewood, P. (2004). Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques. In: Aguilar, MI. (eds) HPLC of Peptides and Proteins. Methods in Molecular Biology™, vol 251. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-742-4:307

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  • DOI: https://doi.org/10.1385/1-59259-742-4:307

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-977-3

  • Online ISBN: 978-1-59259-742-0

  • eBook Packages: Springer Protocols

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