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HPLC of Peptides and Proteins pp 143–164Cite as

Capillary Separations

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 251))

Abstract

Capillary liquid chromatography (capLC) utilizes columns with inner diameters below 0.5 mm and flow rates from some few μL/min down to the μL/min range. Miniaturization of chromatographic procedures using capLC offers substantial advantages over conventional LC separation methods. Capillary columns show increased separation efficiency, minimal solvent consumption, higher peak concentration at the detector, and higher peptide recovery. Several reviews on the state of the art can be found in the literature refs. 1–4.

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Author information

Authors and Affiliations

  1. Structural and Biological Mass Spectrometry Unit Department of Medical Bioanalysis, IIBB-CSIC, IDIBAPS, Barcelona, Spain

    Montserrat Carrascal & Joaquin Abian

Authors
  1. Montserrat Carrascal
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  2. Joaquin Abian
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Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia

    Marie-Isabel Aguilar

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© 2004 Humana Press Inc.

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Carrascal, M., Abian, J. (2004). Capillary Separations. In: Aguilar, MI. (eds) HPLC of Peptides and Proteins. Methods in Molecular Biology™, vol 251. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-742-4:143

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  • DOI: https://doi.org/10.1385/1-59259-742-4:143

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-977-3

  • Online ISBN: 978-1-59259-742-0

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