Immobilized Metal Ion Affinity Chromatography of Proteins
Immobilized metal ion affinity chromatography (IMAC) of proteins is a high-performance liquid chromatography (HPLC) technique. It has the ability to differentiate a single histidine residue on the surface of a protein (1), it can bind proteins with dissociation constants of 10−5 to 10−7 (2), and has had wide application in the field of molecular biology for the rapid purification of recombinant proteins.
KeywordsHPLC Urea EDTA Hydroxide Carboxyl
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