Immobilized Metal Ion Affinity Chromatography of Proteins

  • Michael Zachariou
Part of the Methods in Molecular Biology™ book series (MIMB, volume 251)


Immobilized metal ion affinity chromatography (IMAC) of proteins is a high-performance liquid chromatography (HPLC) technique. It has the ability to differentiate a single histidine residue on the surface of a protein (1), it can bind proteins with dissociation constants of 10−5 to 10−7 (2), and has had wide application in the field of molecular biology for the rapid purification of recombinant proteins.


Target Protein Stationary Support Linear Velocity Equilibration Buffer Iminodiacetic Acid 
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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Michael Zachariou
    • 1
  1. 1.School of Engineering and ScienceSwinburne UniversityHawthornAustralia

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