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Immobilized Metal Ion Affinity Chromatography of Proteins

  • Michael Zachariou
Part of the Methods in Molecular Biology™ book series (MIMB, volume 251)

Abstract

Immobilized metal ion affinity chromatography (IMAC) of proteins is a high-performance liquid chromatography (HPLC) technique. It has the ability to differentiate a single histidine residue on the surface of a protein (1), it can bind proteins with dissociation constants of 10−5 to 10−7 (2), and has had wide application in the field of molecular biology for the rapid purification of recombinant proteins.

Keywords

Target Protein Stationary Support Linear Velocity Equilibration Buffer Iminodiacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Michael Zachariou
    • 1
  1. 1.School of Engineering and ScienceSwinburne UniversityHawthornAustralia

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