Immobilized Metal Ion Affinity Chromatography of Proteins
Immobilized metal ion affinity chromatography (IMAC) of proteins is a high-performance liquid chromatography (HPLC) technique. It has the ability to differentiate a single histidine residue on the surface of a protein (1), it can bind proteins with dissociation constants of 10−5 to 10−7 (2), and has had wide application in the field of molecular biology for the rapid purification of recombinant proteins.
KeywordsTarget Protein Stationary Support Linear Velocity Equilibration Buffer Iminodiacetic Acid
- 10.Beitle, R. R. and Ataali, M. M. (1992) Immobilized metal affinity chromatography and related techniques. AlChE Symp. Series. 88, 34–44.Google Scholar
- 16.Zachariou, M. and Hearn, M. T. W. (1992) High performance liquid chromatography of amino acids, peptides and proteins. CXXI. 8-hydroxyquinoline-metal chelate chromatographic support∶an additional mode of selectivity in immobilized metal affinity chromatography. J. Chromatogr. 599, 171–177.CrossRefGoogle Scholar
- 20.Qiagen (1998) The QIAexpressionist A handbook for high-level expression and purification of 6xHis-tagged proteins, p. 66.Google Scholar