A major goal of proteomics is qualitative and quantitative analysis of all the proteins expressed in a cell, tissue, or organism. Changes in protein expression owing to a stimulus or condition are measured in a systematic manner, and are used to elucidate mechanisms of cell function and signaling. A strength of proteomics is that a “shot-gun” approach requiring no prior knowledge of the system is often used and does not assume a model prior to data collection. Therefore, proteomics provides the ability to deal with the complexity of biological systems with minimal experimental bias. The complexity of biological systems arises from the numerous parallel signaling pathways that interact with each other. The ability to monitor many proteins simultaneously yields a global view of protein expression and posttranslation modifications, which is much more informative than monitoring a few proteins. Methods that follow a few proteins and assume a model are more likely to miss interactions and yield biased results.
KeywordsElectrospray Ionization Tandem Mass Spectrometry Laser Desorption Ionization Mass Spectrometry Heptafluorobutyric Acid Capillary HPLC Amino Acid Sequence Information
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