Abstract
Zebrafish (Danio rerio) are a popular vertebrate model system, particularly useful for research in developmental genetics and neurobiology. The adults are easy to obtain and rear; the generation times are only a few months; and large clutches of big embryos are produced (100–1000 per mating). The embryos develop externally and are nearly transparent, facilitating microscopy, experimental manipulations, and screening for morphological mutants. High-density genetic linkage maps have been produced for visible and DNA markers, and the genome is being sequenced. Several useful genetic tools have been developed. Large-scale mutagenesis screens have detected genes involved in morphogenesis and other developmental processes (1,2). These screens involved a classical three-generation crossing strategy to detect recessive lethal mutations revealed in homozygous diploid mutants. Similar screens are done using insertional mutagenesis to facilitate cloning of the disrupted genes (3). Many useful molecular markers of differentiating cells have been characterized, and gene expression can be effectively manipulated by the use of morpholino antisense oligonucleotides or expression of dominant-negative mutant proteins. Much information concerning zebrafish mutants, strains, and methods can be found at http://www.zfin.org.
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Driever, W., Solnica-Krezel, L., Schier, A., Neuhauss, S., Malicki, J., Stemple, D., et al. (1996) A genetic screen for mutations affecting embryogenesis in zebrafish. Development 123, 37–46.
Haffter, P., Granato, M., Brand, M., Mullins, M., Hammerschmidt, M., Kane, D., et al. (1996) The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio. Development 123, 1–36.
Talbot, W. S. and Hopkins, N. (2000) Zebrafish mutations and functional analysis of the vertebrate genome. Genes Devel. 14, 755–762.
Corley-Smith, G. E., Lim, C. J., and Brandhorst, B. P. (1996) Production of androgenetic zebrafish (Danio rerio). Genetics 142, 1265–176.
Postlethwait, J. H., Johnson, S. L., Midson, C. N., Talbot, W. S., Gates, M., et al. (1994) A genetic linkage map for the zebrafish. Science 264, 699–703.
Postlethwait, J. H. (1998) Using random amplified polymorphic DNAs in zebrafish genomic analysis. Meth. Cell Biol. 60, 165–179.
Johnson, S. L., Gates, M. A., Johnson, M., Talbot, W. S., Horne, S., et al. (1996) Centromere-linkage analysis and consolidation of the zebrafish genetic map. Genetics 142, 1277–1288.
Barbazuk, W. B., Korf, I., Kadavi, C., Heyen, J., Tate, S., Wun, E., et al. (2000) The syntenic relationship of the zebrafish and human genomes. Genome Res. 10, 1351–1358.
Singer, A., Perlman, H., Yan, Y. L., Walker, C., Corley-Smith, G., Brandhorst, B., and Postlethwait, J. (2002) Sex-specific recombination rates in zebrafish (Danio rerio) Genetics 160, 649–657.
Kimmel, C. B. (1989) Genetics and early development of zebrafish. Trends Genet. 5, 283–288.
Walker, C. (1999) Haploid screens and gamma-ray mutagenesis. Meth. Cell Biol. 60, 43–86.
Streisinger, G., Walker, C., Dower, N., Knauber, D., and Singer, F. (1981) Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature 291, 293–296.
Streisinger, G., Singer, F., Walker, C., Knauber, D., and Dower, N. (1986) Segregation analysis and gene-centromere distances in zebrafish. Genetics 112, 311–319.
Peligri, F. and Schulte-Merker, S. (1999) A gynogenesis-based screen for maternal effect genes in the zebrafish. Meth. Cell Biol. 60, 1–20.
Beattie, C. E., Raible, D. W., Henion, P. D., and Judith Eisen (1999) Meth. Cell Biol. 60, 71–86.
Gibbs, P. D., Peek, L. A., and Thorgaard, G. (1994) An in vivo screen for the luciferase transgene in zebrafish. Mol. Mar. Biol. Biotech. 3, 307–316.
Corley-Smith, G. E., Brandhorst, B. P., Walker, C., and Postlethwait, J. H. (1999) Production of haploid and diploid androgenetic zebrafish (including methodology for delayed in vitro fertilization). Meth. Cell Biol. 59, 45–60.
Sakai, N., Burgess, S., and Hopkins, N. (1997) Delayed in vitro fertilization of zebrafish eggs in Hank’s saline containing bovine serum albumin. Mol. Mar. Biol. Biotechnol. 6, 84–87.
Hertwig, O. (1911) Die raiumkrankeheit tierischer keimsellen. Arch. Mikr. Anat. 77, 1–97.
Kane, D. A. and Kimmel, C. B. (1993) The zebrafish midblastula transition. Development 119, 447–456.
Johnson, S. L. and Zon, L. I. (1999) Genetic backgrounds and some standard stocks and strains used in zebrafish developmental biology and genetics. Meth. Cell Biol. 60, 357–359.
McCune, A. R., Fuller, R. C., Aquilina, A. A., Dawley, R. M., Fadool, J. M., Houle, D., et al. (2002) A low genomic number of recessive lethals in natural populations of bluefin killifish and zebrafish. Science 296, 2398–2401.
Amores, A. and Postlethwait, J. H. (1999) Banded chromosomes and the zebrafish karyotype. Meth. Cell Biol. 60, 323–338.
Walker, C. and Streisinger, G. (1983) Induction of mutations by gamma-rays in pregonial germ cells of zebrafish embryos. Genetics 103, 125–136.
Endo, A. and Ingalls, T. H. (1968) Chromosomes of the zebrafish, a model for cytogenetic, embryologic, and ecologic study. J. Heredity 59, 382–384.
Fritz, A., Rozowski, M., Walker, C., and Westerfield, M. (1996) Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction. Genetics 144, 1735–1745.
Riley, B. B. and Grunwald, D. J. (1995) Efficient induction of point mutations allowing recovery of specific-locus mutations in zebrafish. Proc. Natl. Acad. Sci. USA 92, 5997–6001.
Ransom, D. G. and Zon, L. I. (1999) Collection, storage, and use of zebrafish sperm. Meth. Cell Biol. 60, 365–372.
Young, W. P., Wheeler, P. A., Coryell, V. H., Keim, P., and Thorgaard, G. H. (1998) A detailed linkage map of rainbow trout produced using doubled haploids. Genetics 148, 839–850.
Black, S. D. and Gerhart, J. C. (1986) High-frequency twinning of Xenopus laevis embryos from eggs centrifuged before first cleavage. Dev. Biol. 116, 228–240.
Elinson, R. P. and Rowning, B. (1988) A transient array of parallel microtubules in frog eggs: potential tracks for a cytoplasmic rotation that specifies the dorso-ventral axis. Dev. Biol. 128, 185–197.
Jesuthasan, S. and Stahle, U. (1997) Dynamic microtubules and specification of the zebrafish embryonic axis. Curr. Biol. 7, 31–42.
Ungar, A. R., Helde, K. A., and Moon, R. T. (1998) Production of androgenetic haploids in zebrafish with ultraviolet light. Mol. Mar. Biol. Biotech. 7, 320–326.
Lin, F. and Dabrowski, K. (1998) Androgenesis and homozygous gynogenesis in muskellunge (Esox masquinongy): evaluation using flow cytometry. Mol. Rep. Devel. 49, 10–18.
McGrath, J. and Solter, D. (1984) Completion of mouse embryogenesis requires both the maternal and paternal genomes. Cell 37, 179–83.
Haig, D. and Trivers, R. The evolution of parental imprinting: a review of hypotheses, in Genomic Imprinting: Causes and Consequences (Ohlsson, R, Hall, K., and Ritzen, M., eds) Cambridge University Press, Cambridge UK), 1995, pp. 17–28.
Corley-Smith, G. E. and Brandhorst, B. P. (1999) Preservation of endangered species and populations: A role for genome banking, somatic cell cloning, and androgenesis? Mol. Rep. Devel. 53, 363–367.
Kercsényi, M., Magyary, I., Urbányi, B., Orbán, L., and Horvath, L. (1998). Hatching out goldfish from common carp eggs: interspecific androgenesis between two cyprinid species. Genome 41, 573–579.
Corley-Smith, G. E., Lim, C. J., Kalmar, G. B., and Brandhorst, B. P. (1997) Efficient detection of DNA polymorphisms by fluorescent RAPD analysis. Biotechniques 22, 690–696.
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Brandhorst, B.P., Corley-Smith, G.E. (2004). Production of Haploid and Diploid Androgenetic Zebrafish. In: Schatten, H. (eds) Germ Cell Protocols. Methods in Molecular Biology™, vol 254. Humana Press. https://doi.org/10.1385/1-59259-741-6:255
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DOI: https://doi.org/10.1385/1-59259-741-6:255
Publisher Name: Humana Press
Print ISBN: 978-1-58829-257-5
Online ISBN: 978-1-59259-741-3
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