Abstract
Rabbits are widely used as experimental animals in various fields of research. However, unlike mice and rats, rabbits with a homologous genetic background are difficult to obtain, as few inbred strains have been established because of the fact that inbreeding decreases reproductive ability. Moreover, an embryonic stem (ES) cell line with germline chimeras has not been established in rabbits; and thus, there are no reports on production of transgenic rabbits by gene targeting.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Onodera, M. and Tsunoda, Y. (1989) Parthenogenetic activation of mouse and rabbit eggs by electric stimulation in vivo. Gamete Res. 22, 277–283.
Ozil, J.-P. (1990) Parthenogenetic development of rabbit oocytes after repetitive pulsatile electrical stimulation. Development 109, 117–127.
Yin, X. J., Tani, Y., Kato, Y., and Tsunoda, Y. (2000) Development of rabbit parthenogenetic oocytes and nuclear-transferred oocytes receiving cultured cumulus cells. Theriogenology 54, 1469–1476.
Ozil, J.-P. and Huneau, D. (2001) Activation of rabbit oocytes: the impact of the Ca2+ signal regime on development. Development 128, 917–918.
Surani, M. A., Barton, S. C., and Norris, M. L. (1984) Development of reconstituted mouse eggs suggests imprinting of the genome during gametogenesis. Nature 308, 548–550.
Yang, X. and Foote, R. H. (1987) Production of identical twin rabbits by micro-manipulation of embryos. Biol. Reprod. 37, 1007–1014.
Tsunoda, Y. and Kato, Y. (2002) Recent progress and problems in animal cloning. Differentiation 69, 158–161.
Stice, S. L. and Robl, J. M. (1988) Nuclear reprogramming in nuclear transplant rabbit embryos. Biol. Reprod. 39, 657–664.
Tsunoda, Y., Maruyama, Y., Kita, M., Ushijima, H., Kono, T., Tokunaga, Y., and Imai, H. (1989) Nuclear transplantation of 8∼16-cell embryos into enucleated oocytes in the rabbit. Jap. J. Zootech. Sci. 60, 846–851.
Collas, P. and Robl, J. M. (1990) Factors affecting the efficiency of nuclear transplantation in the rabbit embryo. Biol. Reprod. 43, 877–884.
Yang, X., Jiang, S.-E., Kovacs, A., and Foote, R. H. (1992) Nuclear totipotency of cultured rabbit morulae to support full-term development following nuclear transfer. Biol. Reprod. 47, 636–643.
Stice, S. L. and Keefer, C. L. (1993) Multiple generational bovine embryo cloning. Biol. Reprod. 48, 715–719.
Takano, H., Koyama, K., Kozai, C., Shimizu, S., Kato, Y., and Tsunoda, Y. (1997) Cloning of bovine embryos by multiple nuclear transfer. Theriogenology 47, 1365–1373.
Wilmut, I., Schnieke, A. E., McWhir, J., Kind, A.J., and Campbell, K. H. (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810–813.
McCreath, K. J., Howcroft, J., Campbell, K. H., Colman, A., Schnieke, A. E., and Kind, A. J. (2000) Production of genetargeted sheep by nuclear transfer from cultured somatic cells. Nature 405, 1066–1069.
Lai, L., Kolber-Simonds, D., Park, K.-W., Cheong, H.-T., Greenstein, J. L., et al. (2002) Production of α-1,3-galactosyl transferase knockout pigs by nuclear transfer cloning. Science 295, 1089–1092.
Dai, Y., Vaught, T. D., Boone, J., Chen, S.-H., Phelps, C. J., Ball, et al. (2002) Targeted disruption of the α-1,3-galactosyltransferase gene in cloned pigs. Nature Biotechnol. 20, 251–255.
Mitalpov, S. M., White, K. L., Farrar, V. R., Morrey, J., and Reed, W. A. (1999) Development of nuclear transfer and parthenogenetic rabbit embryos activated inositol 1,4,5-triphosphate. Biol. Reprod. 60, 821–827.
Yin, X. J., Kato, Y., and Tsunoda, Y. (2002a) Effect of enucleation procedures and maturation conditions on the development of nuclear-transferred rabbit oocytes receiving male fibroblast cells. Reproduction 124, 41–47.
Yin, X. J., Kato, Y., and Tsunoda, Y. (2002b) Effect of delayed enucleation on the developmental potential of nuclear-transferred oocytes receiving adult and fetal fibroblast cells. Zygote 10, 217–222.
Chesne, P., Adenot, P. G., Viglietta, C., Baratte, M., Boulanger, L., and Renard, J.-P. (2002) Cloned rabbits produced by nuclear transfer from adult somatic cells. Nature Biotechnol. 20, 366–369.
Harper, M. J. K. (1963) Ovulation in the rabbit: the time of follicular rapture and expulsion of the eggs, in relation to injection luteinizing hormone. J. Reprod. Fertil. 26, 307–316.
Campbell, K. H. S., Loi, P., Otaegui, P. J., and Wilmut, I. (1996) Cell cycle co-ordination in embryo cloning by nuclear transfer. Rev. Reprod. 1, 40–46.
Tani, T., Kato, Y., and Tsunoda, Y. (2001) Direct exposure of chromosomes to nonactivated ovum cytoplasm is effective for bovine somatic cell nucleus reprogramming. Biol. Reprod. 64, 324–330.
Yin, X. J., Tani, T., Yonemura, I., Kawakami, M., Miyamoto, K., Hasegawa, R., et al. (2002) Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes. Biol. Reprod. 67, 442–446.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc.
About this protocol
Cite this protocol
Tsunoda, Y., Kato, Y. (2004). Parthenogenesis and Nuclear Transfer in Rabbit Oocytes. In: Schatten, H. (eds) Germ Cell Protocols. Methods in Molecular Biology™, vol 254. Humana Press. https://doi.org/10.1385/1-59259-741-6:195
Download citation
DOI: https://doi.org/10.1385/1-59259-741-6:195
Publisher Name: Humana Press
Print ISBN: 978-1-58829-257-5
Online ISBN: 978-1-59259-741-3
eBook Packages: Springer Protocols