Microfluidic Removal of Cumulus Cells from Mammalian Zygotes
Micron-sized fluid channels have been used for biology-related procedures, such as single-cell manipulation (1, 2, 3), cell deformation (4,5), cell assays (6), and subcellular molecule positioning (7). Owing to laminar fluid flow on this scale, small volumes of fluid can be precisely controlled (8,9). Microfluidic tools allow for the control and manipulation on the size scale of a single embryo (10). Precise microfluidic manipulation can be employed to perform multiple IVP-processing steps (11, 12, 13).
KeywordsPolyethylene Syringe Casing
- 1.Ayliffe, H. E., Rabbitt, R. D. Tresco, P. A., and Frazier, A. B. (1997) Micromachined cellular characterization system for studying the biomechanics of individual cells, in Transducers 97. International Conference on Solid-State Sensors and Actuators. Chicago, IL, IEEE, 1997.Google Scholar
- 6.Andersson, P. E., Li, P. C. H., Smith, R., Szarka, R. J., and Harrison, D. J. Biological cell assays on an electrokinetic microchip, in (1997 Int’l Conf. on Solid-State Sensors and Actuators. Chicago, IL, 1997.Google Scholar
- 11.Raty, S., Davis, J. A., Beebe, D. J., Rodriguez-Zas, S. L., and Wheeler, M. B. (2001) Culture in microchannels enhance in vitro embryonic development of pre-implantation mouse embryos. Theriogenology 55, 241.Google Scholar
- 13.Zeringue, H. C., King, K. R., Glasgow, I. K., Raty, S., Wheeler, M. B. and Beebe, D. J. Zona pellucida removal of mammalian embryos in a microfluidic system, in Micro Total Analysis Systems. Enschede, the Netherlands, 2000.Google Scholar