Microfluidic Removal of Cumulus Cells from Mammalian Zygotes
Micron-sized fluid channels have been used for biology-related procedures, such as single-cell manipulation (1, 2, 3), cell deformation (4,5), cell assays (6), and subcellular molecule positioning (7). Owing to laminar fluid flow on this scale, small volumes of fluid can be precisely controlled (8,9). Microfluidic tools allow for the control and manipulation on the size scale of a single embryo (10). Precise microfluidic manipulation can be employed to perform multiple IVP-processing steps (11, 12, 13).
KeywordsPressure Fluctuation Cumulus Cell Plunger Movement Laminar Fluid Flow Removal Port
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