Abstract
Confocal microscopy is routinely used to produce high-resolution images of single, double-, and triple labeled fluorescent samples. The images are collected as single optical sections (2D imaging), as Z-series (3D imaging), as time-lapse series (2D over time), or as Z-series over time (3D over time or 4D imaging). Because the images are in a digital format, they can be further manipulated using a range of software.
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References
White N. S. (1995) Visualization systems for multidimensional CLSM images, in Handbook of Biological Confocal Microscopy, Plenum Press, New York.
Thomas, C., DeVries, P., Hardin, J., and White, J. (1996) Four-dimensional imaging: computer visualization of 3D movements in living specimens. Science 273, 603–607.
Mohler, W. A., and White, J. G. (1998) Stereo-4-D reconstruction and animation from living fluorescent specimens. BioTechniques 24, 1006–1012.
Schrock, E., du Manoir, S., Veldman, T., Schoell, B., Wienberg, J., Ferguson-Smith, M. A., Ning, Y., Ledbetter, D. H., Bar-Am, I., Soenksen, D., Garini, Y., Ried, T. (1996) Multicolor spectral karyotyping of human chromosomes. Science 273, 494–497.
Waggoner, A. S., DeBiasio, R., Conrad, P., Bright, G. R., Ernst, L. A., Ryan, K., Nederlof, M., Taylor, D. L. (1989) Multiple spectral parameter imaging. Methods in Cell Biol. 30, 449–478.
Paddock, S. W., Langeland, J. A., DeVries, P. J., and Carroll, S. B. (1993) Three-color immunofluorescence imaging of Drosophila embryos by laser scanning confocal microscopy. BioTechniques 14, 42–48.
Kiehart, D. P., Montague, R. A., Rickoll, W. L., Thomas, G. H., and Foard, D. (1994) High-resolution microscopic methods for the analysis of cellular movements in Drosophila embryos. Methods Cell Biol. 44, 507–532.
Paddock, S. W., DeVries, P. J., Buth, E., and Carroll, S. B. (1994) Morphing: a new graphics tool for animating confocal images. BioTechniques 16, 448–452.
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© 1999 Humana Press Inc., Totowa, NJ
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Halder, G., Paddock, S.W. (1999). Presentation of Confocal Images. In: Paddock, S.W. (eds) Confocal Microscopy Methods and Protocols. Methods in Molecular Biology™, vol 122. Humana Press. https://doi.org/10.1385/1-59259-722-X:373
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DOI: https://doi.org/10.1385/1-59259-722-X:373
Publisher Name: Humana Press
Print ISBN: 978-0-89603-526-3
Online ISBN: 978-1-59259-722-2
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