Abstract
Confocal laser scanning microscopy (CLSM) can be used to obtain optical sections of thick tissues that are relatively free of interfering autofluorescence, and that do not strongly scatter or absorb either the excitation or emission light. This chapter provides protocols used to examine three such tissues: the cornea of the eye, the buccal mucosa (which lines the inner cheek), and the nasal respiratory epithelium. Although in each case our overall motivation was to study the transport of drugs or model compounds across the particular epithelium, the approaches taken were quite different.
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© 1999 Humana Press Inc., Totowa, NJ
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Imbert, D., Hoogstraate, J., Marttin, E., Cullander, C. (1999). Imaging Thick Tissues with Confocal Microscopy. In: Paddock, S.W. (eds) Confocal Microscopy Methods and Protocols. Methods in Molecular Biology™, vol 122. Humana Press. https://doi.org/10.1385/1-59259-722-X:341
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DOI: https://doi.org/10.1385/1-59259-722-X:341
Publisher Name: Humana Press
Print ISBN: 978-0-89603-526-3
Online ISBN: 978-1-59259-722-2
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