Imaging Thick Tissues with Confocal Microscopy

  • Delphine Imbert
  • Janet Hoogstraate
  • Emmeline Marttin
  • Christopher Cullander
Part of the Methods in Molecular Biology™ book series (MIMB, volume 122)


Confocal laser scanning microscopy (CLSM) can be used to obtain optical sections of thick tissues that are relatively free of interfering autofluorescence, and that do not strongly scatter or absorb either the excitation or emission light. This chapter provides protocols used to examine three such tissues: the cornea of the eye, the buccal mucosa (which lines the inner cheek), and the nasal respiratory epithelium. Although in each case our overall motivation was to study the transport of drugs or model compounds across the particular epithelium, the approaches taken were quite different.


Confocal Laser Scanning Microscopy Nasal Cavity Buccal Mucosa Evans Blue Probe Solution 
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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Delphine Imbert
    • 1
  • Janet Hoogstraate
    • 2
  • Emmeline Marttin
    • 3
  • Christopher Cullander
    • 4
  1. 1.Cellegy Pharmaceutical, Inc.Foster City
  2. 2.Astra Pain Control ABSodertaljeSweden
  3. 3.Leiden-Amsterdam Center for Drug ResearchUniver?sity of LeidenThe Netherlands
  4. 4.Department of Biopharmaceutical Sciences, School of PharmacyUniversity of CaliforniaSan Francisco

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