Abstract
From the clinical perspective, steroids have always held a great deal of interest, since they are highly specific in their function. They do not have a general or systemic effect, but instead regulate specific physiological functions, such as sex differentiation, fetus implantation and growth, electrolyte balance, menstrual cycles, and muscle and bone development. Many disorders have been identified as being caused by under- or oversecretion of steroids, i.e., Addison’s disease, Cushing’s syndrome, hirsutism and virilism, adenomas, congenital adrenal hyperplasia, acromegaly, Liddle’s syndrome, hypertension, and so on (1). Diseases resulting from steroid imbalance usually result from the cumulative effect of one or more steroids. To get a better understanding of the patho-physiology resulting from steroid imbalances, the measurement of a profile of steroids is potentially more beneficial than measuring a single steroid. Fiet et al. (2), who used a profile of eight steroids to gain a better understanding of hirsutism and acne in women, demonstrated a good example of this type of study.
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References
Greenspan, F. S. (ed.) (1991) Basic and Clinical Endocrinology, 3rd ed. Appleton and Lange, CA.
Fiet, J., Gosling, J. P., Soliman, H., Galons, H., and Vexiau, P. (1994) Hirsutism and acne in women: coordinated radioimmunoassays for eight relevant plasma steroids. Clin. Chem. 40, 2296–2305.
Bosworth, N. and Towers, P. (1989) Scintillation proximity assay. Nature 341, 167–168.
Gueux, B., Fiet, J., Pham-Huu-Trung, Villette, J. M., Gourmelen, M., Galons, H., et al. (1985) Radioimmunoassay for 20-deoxy cortisol: clinical application. Acta Endocrinal (Copenhagen) 108, 537–544.
Fiet, J., Villette, J. M., Galons, H., Boudou, P., Burthier, J. M., and Hardy, N. (1994) Application of new highly sensitive radioimmunoassay for plasma 21-deoxycortisol to the detection of steroid 21-hydroxylase deficiency. Ann. Clin. Biochem. 31, 51–64.
Marx, J. L. (1978) Estrogens: hormones link to cancer disputed. Science 202, 1270–1271.
Rudiger, H. W., Haenigsch, F., Metzler, M., Oesch, F., and Glatt, H. R. (1979) Metabolites of diethylstilboesterol induced sister chromatid exchange in human cultured fibroblasts. Nature 281, 392–394.
Donike, M. and Zimmermann, J. (1980) Zur darstellung von Trimethylsilyl-, triethysilyl-und tert-butyldimethylsilyl-enolthern von ketosteroiden fr gas-chromato-graphische und massenspektrometrische untersuchungen. J. Chromatogr. 202, 483.
Nishi, H., Fukuyama, T., Matsuo, M., and Terabe, S. (1990) Separation and determination of lipophilic corticosteroids and benzothiazepin analogues by micellar electrokinetic chromatography using bile salts. J. Chromatogr. 513, 279–295.
Bumgarner, J. G. and Khaledi, M. G. (1996) J. Chromatogr. B. 674, 275.
Mohammad, A. A., Bissell, M. G., and Petersen, J. R. (1995) Rapid profiling of clinically relevant steroids by micellar electrokinetic capillary chromatography. J. Capillary Electrophoresis 2, 105–110.
Valbuena, G. A., Rao, L. V., Petersen, J. R., Okorodudu, A. O., Bissell, M. G., and Mohammad, A. A. (1997) Anionic-zwitterionic mixed micelles in micellar electro-kinetic separation of clinically relevant steroids. J. Chromatogr. A. 781, 467–474.
Mohammad, A. A., Petersen, J. R., and Bissell, M. G. (1995) Micellar electrokinetic capillary chromatographic separation of steroids in urine by trioctylphosphine oxide and cationic surfactant. J. Chromatogr. B. 674, 31–38.
Tsuda, T. (1987) J. High Resolut. Chromatogr. Commun. 10, 622.
Tietz, N. W. (ed.) (1995) Clinical Guide to Laboratory Tests, 3rd ed. Saunders, Philadelphia, PA.
Mohammad, A. A., Petersen, J. R., and Bissell, M. G. (1995) Micellar electrokinetic capillary chromatography to separate steroids that are increased in congenital adrenal hyperplasia. Clin. Chem. 41, 1369–1370.
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© 1999 Humana Press Inc.
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Mohammad, A.A., Petersen, J.R., Bissell, M.G. (1999). Steroid Analysis by Micellar Electrokinetic Capillary Chromatography. In: Palfrey, S.M. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Medicine™, vol 27. Humana Press. https://doi.org/10.1385/1-59259-689-4:177
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DOI: https://doi.org/10.1385/1-59259-689-4:177
Publisher Name: Humana Press
Print ISBN: 978-0-89603-639-0
Online ISBN: 978-1-59259-689-8
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