Abstract
Electrophoresis is an important and fundamental tool for DNA analysis. Traditional DNA electrophoresis is labor-intensive, skill-dependent, and relatively slow. To achieve greater performance, capillary electrophoresis (CE) has been developed for DNA work (1–2). The DNA fragments can be separated by molecular sieving in CE with gel-filled capillaries, based on the same principle as that of the conventional gel electrophoresis. The whole procedure can be completed in a relatively short period with great resolution. Recently, CE equipped with laser-induced fluorescence (CE-LIF) has been used as a tool for DNA analysis (3). Using fluorescence DNA intercalators in a gel-filled capillary, the detection sensitivity was found to increase 2–3 orders of magnitude over that of UV detection, and reaches picogram levels (3).
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© 1999 Humana Press Inc.
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Liu, MS., Chen, FT.A. (1999). Rapid Analysis of Amplified Double-Stranded DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection. In: Palfrey, S.M. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Medicine™, vol 27. Humana Press. https://doi.org/10.1385/1-59259-689-4:121
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DOI: https://doi.org/10.1385/1-59259-689-4:121
Publisher Name: Humana Press
Print ISBN: 978-0-89603-639-0
Online ISBN: 978-1-59259-689-8
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