Skip to main content
SpringerLink
Log in

DNA Fusion Vaccines Against B-Cell Tumors

  • Protocol
DNA Vaccines

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 29))

  • 816 Accesses

Abstract

The ability of naked DNA to induce immune responses against encoded antigen has been clearly demonstrated for infectious diseases (1). In many cases, the induced immunity is able to protect against infection, and can approach the efficacy of exogenous antigen (2).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Ulmer, J. B., Donnelly, J. J., Parker, S. E., Rhodes, G. H., Felgner, P. L., Dwarki, V. J., Gromkowski, S. H., Deck, R. R., DeWitt, C. M., Friedman, A., Hawe, L. A., Leander K. R., Martinez, D., Perry, H. C., Shiver, J. W., Montgomery, D. L., and Liu, M. A. (1993) Heterologous protection against influenza by injection of DNA encoding a viral protein. Science 259, 1745–1749.

    Article  PubMed  CAS  Google Scholar 

  2. Davis, H. L. and Whalen, R. G. (1995) DNA-based immunization. In Molecular and Cell Biology of Human Gene Therapeutics. (Dickson, G., ed.) Chapman & Hall, New York, p. 368.

    Google Scholar 

  3. Bogen, B. (1996) Peripheral T cell tolerance as a tumor escape mechanism: deletion of CD4+ T cells specific for a monoclonal immunoglobulin idiotype secreted by a plasmacytoma. Eur. J. Immunol. 26, 2671–2679.

    Article  PubMed  CAS  Google Scholar 

  4. Spellerberg, M. B., Zhu, D., Thompsett, A., King, C. A., Hamblin, T. J., and Stevenson, F. K. (1997) DNA vaccines against lymphoma: Promotion of anti-idiotypic antibody responses induced by single chain Fv gene by fusion to tetanus toxin fragment C. J. Immunol. 159, 1885–1892.

    PubMed  CAS  Google Scholar 

  5. Stevenson, F. K., Zhu, D., King, C. A., Ashworth, L. J., Kumar, S., and Hawkins, R. E. (1995) Idiotypic vaccines against B-cell lymphoma. Immunol. Rev. 145, 211–228.

    Article  PubMed  CAS  Google Scholar 

  6. George, A. J. T., and Stevenson, F. K. (1989) Prospect for the treatment of B cell tumors using idiotypic vaccination. Int. Rev. Immunol. 4, 271–310.

    Article  PubMed  CAS  Google Scholar 

  7. George, A. J. T. Tutt, A. L., and Stevenson, F. K. (1987) Anti-idiotypic mechanisms involved in suppression of a mouse B cell lymphoma, BCL1. J. Immunol. 138, 628–634.

    PubMed  CAS  Google Scholar 

  8. Kaminski, M. S., Kitamura, K., Maloney, D. G., and Levy, R. (1987) Idiotypic vaccination against a murine B cell lymphoma: inhibition of tumor immunity by free idiotypic protein. J. Immunol. 138, 1289–1296.

    PubMed  CAS  Google Scholar 

  9. Hawkins, R. E., Zhu, D., Ovecka, M., Winter, G., Hamblin, T. J. Long, A., and Stevenson, F. K. (1994) Idiotypic vaccination against human B-cell lymphoma: rescue of variable region gene sequences from biopsy material for assembly as single-chain Fv personal vaccines. Blood 83, 3279–3288.

    PubMed  CAS  Google Scholar 

  10. Winter, G., Griffiths, A. D., Hawkins, R. E., and Hoogenboom, H. R. (1994) Making antibodies by phage display technology. Ann. Rev. Immunol. 12, 433–455.

    Article  CAS  Google Scholar 

  11. Sahota, S. S., Leo, R., Hamblin, T. J., and Stevenson, F. K. (1996) IgVH gene mutational patterns indicate different tumor cell status in human myeloma and monoclonal gammopathy of undetermined significance. Blood 87, 746–755.

    PubMed  CAS  Google Scholar 

  12. Levy, R., Levy, S., Cleary, M. L., Carroll, W., Kon, S., Bird, J., and Sklar, J. (1987) Somatic mutation in human B-cell tumors. Immunol. Rev. 96, 43–58.

    Article  PubMed  CAS  Google Scholar 

  13. Panina-Bordignon, P., Tan, A., Termitjelen, A., Demotz, S., Corradin, G., and Lanzavecchia, A. (1989) Universally immunogenic T cell epitopes: promiscuous binding to human MHC class II and promiscuous recognition by T cells. Eur. J. Immunol. 19, 2237–2242.

    Article  PubMed  CAS  Google Scholar 

  14. Anderson, R., Gao, X.-M., Papakonstantinopoulou, A., Roberts, M., and Dougan, cal Protocol: A pilot study of idiotypic vaccination for follicular B-cell lymphoma using a genetic approach. Hum. Gene Ther. 8, 1287–1299.

    Google Scholar 

  15. Manthorpe, M., Cornefert-Jensen, F., Hartikka, J., Felgner, J., Rundell, A., Margalith, M., and Dwarki, V. (1993) Gene therapy by intramuscular injection of plasmid DNA: studies on firefly luciferase gene expression in mice. Hum. Gene Ther. 4, 419–431.

    Article  PubMed  CAS  Google Scholar 

  16. Pascual, V., Victor, K., Spellerberg, M., Hamblin, T. J., Stevenson, F. K., and Capra, J. D. (1992) VH restriction among human cold agglutinins. The VH-21 gene segment is required to encode anti-I and anti-i specificities. J. Immunol. 149, 2337–2344.

    PubMed  CAS  Google Scholar 

  17. Vollmers, H. P., Wozniak, E., Stepien-Bötsch, E., Zimmermann, U., and Müller-Hermelink, H.-K. (1996) A rapid method for purification of monoclonal human IgM from mass culture. Hum. Antibodies Hybridomas 7, 37–41.

    PubMed  CAS  Google Scholar 

  18. Sato, Y., Roman, M. Tighe, H., Lee, D., Corr, M., Nguyen, M.-D., Silverman, G. J., Lotz, M., Carson, D. A., and Raz, E. (1996) Immunostimulatory DNA sequences necessary for effective intradermal gene immunization. Science 273, 352–354.

    Article  PubMed  CAS  Google Scholar 

  19. Dyke, R. J., McBride, H., George, A. J. T., Hamblin, T. J., and Stevenson, F. K. (1991) Idiotypic vaccination against B-cell lymphoma leads to dormant tumour. Cell Immunol. 132, 70–83.

    Article  PubMed  CAS  Google Scholar 

  20. King, C. A., Spellerberg, M. B., Zhu, D., Rice, J., Sahota, S. S., Thompsett, A. R., Hamblin, T. J., Radl, J., and Stevenson, F. K. (1998) DNA vaccines with single-chain Fv fused to fragment C of tetanus toxin induce protective immunity against lymphoma and myeloma. Nat. Med. 4, 1281–1286.

    Article  PubMed  CAS  Google Scholar 

  21. Sambrook, J., Fritch, E. F., and Maniatis, T. (1989) Molecular Cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Immunology Group, Tenovus Research Laboratory, Southampton University Hospitals Trust, Southampton, UK

    Delin Zhu, Myfanwy B. Spellerberg, Catherine A. King, Jason Rice, Andrew R. Thompsett & Freda K. Stevenson

Authors
  1. Delin Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Myfanwy B. Spellerberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Catherine A. King
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jason Rice
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Andrew R. Thompsett
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Freda K. Stevenson
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Mycobacterial Research Laboratory, London, UK

    Douglas B. Lowrie MD

  2. Maxygen Inc., Redwood City, CA

    Robert G. Whalen MD

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Zhu, D., Spellerberg, M.B., King, C.A., Rice, J., Thompsett, A.R., Stevenson, F.K. (2000). DNA Fusion Vaccines Against B-Cell Tumors. In: Lowrie, D.B., Whalen, R.G. (eds) DNA Vaccines. Methods in Molecular Medicine™, vol 29. Humana Press. https://doi.org/10.1385/1-59259-688-6:405

Download citation

  • DOI: https://doi.org/10.1385/1-59259-688-6:405

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-580-5

  • Online ISBN: 978-1-59259-688-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation