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Immunization with Naked DNA Coexpressing Antigen and Cytokine via IRES

  • Protocol
DNA Vaccines

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 29))

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Abstract

Inoculation of plasmid DNA vectors encoding immunogenic proteins induce humoral as well as cell-mediated immune responses. Protection against challenge with pathogens has provided protective immunity in several instances in animal models. (1,2). DNA vaccines allow the simultaneous expression of antigens and immune-stimulatory cytokines via an internal ribosomal entry site (IRES). Here we describe the construction of a DNA vaccine against malignant melanomas using: (i) the tumor-associated antigen gp100 (or pmel17), known to be over-expressed in many malignant melanomas (3,4), and (ii) the granulocyte macrophage stimulating factor (GM-CSF) which has been shown to have a stimulatory effect on humoral and cellular immune responses (5).

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Author information

Authors and Affiliations

  1. Institute of Medical Virology, University of Zurich, Zurich, Switzerland

    Jochen Heinrich, Bettina Strack, Michael Nawrath, Jovan Pavlovic & Karin Moelling

Authors
  1. Jochen Heinrich
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  2. Bettina Strack
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  3. Michael Nawrath
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  4. Jovan Pavlovic
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  5. Karin Moelling
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Editor information

Editors and Affiliations

  1. Mycobacterial Research Laboratory, London, UK

    Douglas B. Lowrie MD

  2. Maxygen Inc., Redwood City, CA

    Robert G. Whalen MD

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© 2000 Humana Press Inc., Totowa, NJ

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Heinrich, J., Strack, B., Nawrath, M., Pavlovic, J., Moelling, K. (2000). Immunization with Naked DNA Coexpressing Antigen and Cytokine via IRES. In: Lowrie, D.B., Whalen, R.G. (eds) DNA Vaccines. Methods in Molecular Medicine™, vol 29. Humana Press. https://doi.org/10.1385/1-59259-688-6:335

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  • DOI: https://doi.org/10.1385/1-59259-688-6:335

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-580-5

  • Online ISBN: 978-1-59259-688-1

  • eBook Packages: Springer Protocols

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