Abstract
The use of DNA vectors to elicit an immune response has produced a lot of interest. Unfortunately, one of the limiting factors has been the problem of gene expression. In order to obtain a strong expression of the vaccinating gene, several steps are necessary. The vector has to be delivered in such a way that it is not being degraded by the immune nor by the hepatic system; it has also to enter efficiently the targeted cells; and it must be expressed in the appropriate compartment of the cells at a high level. For these reasons, we have developed a gene expression vector that contains a T7 autogene and is being expressed in the cytoplasm of the cells (1,2). We will describe this system and two possible applications: infectious disease vaccination and tumor ablation. The latter application may be combined with DNA vaccination against cancer cells.
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© 2000 Humana Press Inc., Totowa, NJ
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Sturtz, F.G., Li, Y., Shulok, J., Snodgrass, H.R., Chen, Xz. (2000). A Nonviral Cytoplasmic T7 Autogene System and Its Applications in DNA Vaccination. In: Lowrie, D.B., Whalen, R.G. (eds) DNA Vaccines. Methods in Molecular Medicine™, vol 29. Humana Press. https://doi.org/10.1385/1-59259-688-6:323
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DOI: https://doi.org/10.1385/1-59259-688-6:323
Publisher Name: Humana Press
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