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The Use of Conventional Immunologic Adjuvants in DNA Vaccine Preparations

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DNA Vaccines

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 29))

Abstract

The term “adjuvant” originates from the Latin word adjuvare, which means to help or aid. An immunologic adjuvant is defined as any substance that acts to accelerate, prolong, or enhance antigen-specific immune responses when used in combination with specific vaccine antigens (1). In a broad sense, immunologic adjuvants include certain cytokines and other immunomodulatory molecules (i.e. chemokines and costimulatory factors) or their expression vectors. Since these adjuvants are described elsewhere in this book, here, we are concerned only with adjuvants that are derived from microorganisms and plants or are synthesized chemically.

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References

  1. Vogel, F. R. (1995) Immunologic adjuvants for modern vaccine formulations, in Combined Vaccine and Simultaneous Administration—Current Issues and Perspectives, Ann. NY Acad. Sci., vol. 754 (Williams, J. C., Goldenthal, K. L., Burns, D. L., and Lewis, B. P. J., eds.) N Y Academy of Sciences, New York. pp. 153–160.

    Google Scholar 

  2. Powell, M. F. and Newman, M. J., eds. (1995) Vaccine Design: The Subunit and Adjuvant Approach, Plenum Press, New York.

    Google Scholar 

  3. Golding, B. and Scott, D. E. (1995) Vaccine Strategies: Targetting helper T cell responses, in Combined Vaccine and Simultaneous Administration—Current Issues and Perspectives, Ann. NY Acad. Sci. vol. 754 (Williams, J. C., Goldenthal, K. L., Burns, D. L., and Lewis, B. P. J., eds.) NY Academy of Sciences, New York. pp. 126–137.

    Google Scholar 

  4. Gupta, R. K. and Siber, G. R. (1995) Adjuvants for human vaccines-current status, problems and future prospects. Vaccine 13, 1263–1276.

    Article  PubMed  CAS  Google Scholar 

  5. Corr, M., Lee, D. J., Carson, D. A., and Tighe, H. (1996) Gene vaccination with naked plasmid DNA: Mechanism of CTL priming. J. Exp. Med. 184, 1555–1560.

    Article  PubMed  CAS  Google Scholar 

  6. Doe, B., Selby, M., Barnett, S., Baenziger, J., and Walker, C. M. (1996) Induction of cytotoxic T lymphocytes by intramuscular immunization with plasmid DNA is facilitated by bone marrow-derived cells. Proc. Natl. Acad. Sci. USA 93, 8578–8583.

    Article  PubMed  CAS  Google Scholar 

  7. Tsuji, T., Fukushima, J., Hamajima, K., Ishii, N., Aoki, I., Xin, K.-Q., et al. (1997) HIV-1-specific cell-mediated immunity is enhanced by co-inoculation of TCA3 expression plasmid with DNA vaccine. Immunology 90, 1–6.

    Article  PubMed  CAS  Google Scholar 

  8. Sasaki, S., Tsuji, T., Hamajima, K., Fukushima, J., Ishii, N., Kaneko, T., et al. (1997) Monophosphoryl lipid A enhances both humoral and cell-mediated immune responses to DNA vaccination against Human Immunodeficiency Virus Type 1. Infect. Immun. 65, 3520–3528.

    PubMed  CAS  Google Scholar 

  9. Sasaki, S., Sumino, K., Hamajima, K., Fukushima, J., Ishii, N., Kawamoto, S., et al. (1998) Induction of systemic and mucosal immune responsees to human immunodeficiency virus type 1 by a DNA vaccine formulated with QS-21 saponin adjuvant via intramuscular and intranasal routes. J. Virol. 72, 4931–4939.

    PubMed  CAS  Google Scholar 

  10. Kuklin, N., Daheshia, M., Karem, K., Manickan, E., and Rouse, B. T. (1997) Induction of mucosal immunity against herpes simplex virus by plasmid DNA immunization. J. Virol. 71, 3138–3145.

    PubMed  CAS  Google Scholar 

  11. Watanabe, A., Raz, E., Kohsaka, H., Tighe, H., Baird, S. M., Kipps, T. J., and Carson, D. A. (1993) Induction of antibodies to a kappa V region by gene immunization. J. Immunol. 151, 2871–2876.

    PubMed  CAS  Google Scholar 

  12. Xiang, Z. Q., and Ertl, H. C. J. (1995) Manipulation of the immune response to a plasmid-encoded viral antigen by coinoculation with plasmids expressing cytokines. Immunity 2, 129–135.

    Article  PubMed  CAS  Google Scholar 

  13. Tsuji, T., Hamajima, K., Fukushima, J., Xin, K.-Q., Ishii, N., Aoki, I. (1997) Enhancement of cell-mediated immunity against HIV-1 induced by coinoculation of plasmid-encoded HIV-1 antigen with plasmid expressing IL-12. J. Immunol. 158, 4008–4014.

    PubMed  CAS  Google Scholar 

  14. Iwasaki, A., Stiernholm, B. J. N., Chan, A. K., Berinstein, N. L., and Barber, B. H. (1997) Enhanced CTL responses mediated by plasmid DNA immunogens encoding costimulatory molecules and cytokines. J. Immunol. 158, 4591–4601.

    PubMed  CAS  Google Scholar 

  15. Gregoriadis, G., Saffie, R., and de Souza, J. B. (1997) Liposome-mediated DNA vaccination. FEBS Lett. 402, 107–110.

    Article  PubMed  CAS  Google Scholar 

  16. Ishii, N., Fukushima, J., Kaneko, T., Okada, E., Tani, K., Tanaka, S.-I., et al. (1997) Cationic liposomes are a strong adjuvant of DNA vaccine for a Human Immunodeficiency Virus Type-1 (HIV-1). AIDS Res. Hum. Retroviruses 13, 1421–1428.

    Article  PubMed  CAS  Google Scholar 

  17. Gao, X. and Huang, L. (1991) A novel cationic liposome reagent for efficient transfection of mammalian cells. Biochem. Biophys. Res. Comm. 179, 280–285.

    Article  PubMed  CAS  Google Scholar 

  18. Toda, S., Ishii, N., Okada, E., Kusakabe, K.-I., Arai, H., Hamajima, K., et al. (1997) HIV-1 specific cell-mediated immune responses induced by DNA vaccination are enhanced by mannan-coated liposomes and inhibited by anti-inter-feron (IFN)-gamma antibody. Immunology 92, 111–117.

    Article  PubMed  CAS  Google Scholar 

  19. Sasaki, S., Fukushima, J., Arai, H., Kusakabe, K., Hamajima, K., Ishii, N., et al. (1997) Human immunodeficiency virus type 1 specific immune response induced by DNA vaccination are greatly enhanced by mannan-coated diC14-amidine. Eur. J. Immunol. 27, 3121–3129.

    Article  PubMed  CAS  Google Scholar 

  20. Sugimoto, M., Ohishi, K., Fukasawa, M., Shikata, K., Kawai, H., Itakura, H., et al. (1995) Oligomannose-coated liposomes as an adjuvant for the induction of cell-mediated immunity. FEBS Lett. 363, 53–56.

    Article  PubMed  CAS  Google Scholar 

  21. Wang, S., Manning, S., Kensil, C. R., and Lu, S. (1997) QS-21 is effective in boosting anti-HIV-1 Env antibody responses induced by DNA immunization. 9th Annual Meeting of The National Cooperative Vaccine Development Groups for AIDS, poster abstract 15. Bethesda, MD, USA.

    Google Scholar 

  22. Sasaki, S., Hamajima, K., Fukushima, J., Tsuji, T., Xin, K.-Q., Ishii, N., et al. (1997) Adjuvant effect of ubenimex on a DNA vaccine for human immunodeficiency virus type-1. Clin. Exp. Immunol. 111, 30–35.

    Article  Google Scholar 

  23. Hamajima, K., Sasaki, S., Fukushima, J., Kaneko, T., Xin, K.-Q., Kudo, I., and Okuda, K. (1998) Intranasal administration of HIV-DNA vaccine formulated with a polymer, carboxymethyl cellulose augment mucosal antibody production and cell-mediated immune response. Clin. Immunol. Immunopathol. 80, 205–210.

    Article  Google Scholar 

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© 2000 Humana Press Inc., Totowa, NJ

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Sasaki, S., Okuda, K. (2000). The Use of Conventional Immunologic Adjuvants in DNA Vaccine Preparations. In: Lowrie, D.B., Whalen, R.G. (eds) DNA Vaccines. Methods in Molecular Medicine™, vol 29. Humana Press. https://doi.org/10.1385/1-59259-688-6:241

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  • DOI: https://doi.org/10.1385/1-59259-688-6:241

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-580-5

  • Online ISBN: 978-1-59259-688-1

  • eBook Packages: Springer Protocols

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