DNA Vaccines pp 375-395 | Cite as

Genetic Vaccination Targeting T-Cell Receptors

  • Alexis P. Godillot
  • Qiong Fang
  • Terry Higgins
  • Catherine Pachuk
  • Jean Boyer
  • David B. Weiner
  • Stuart Lessin
  • William V. Williams
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 29)


T-cell antigen receptor (TCR) genes (which consist of variable (V), diversity (D), joining (J) and constant (C) segments) undergo rearrangement during T-cell development and result in the expression of a disulfide linked heterodimer (α and ß chains) on the surface of mature T-cells (1,2). The TCR confers specificity to each T-cell for antigen recognition (in the context of major histocompatibility (MHC) molecules (1,3). Clonal TCR-ß chain gene rearrangements have been demonstrated in DNA samples derived from cutaneous tumors, peripheral blood lymphocytes and lymph nodes of patients with cutaneous T cell lymphomas (CTCL) (4, 5, 6). Together with immunohistologic data (7), these findings indicate that CTCL is a clonal disease of malignant T cells that express α/ß-TCR.


Peptide Mixture Tritiated Thymidine Calf Intestinal Alkaline Phosphatase Sodium Perborate Chain Gene Rearrangement 
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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Alexis P. Godillot
    • 1
  • Qiong Fang
    • 1
  • Terry Higgins
    • 2
  • Catherine Pachuk
    • 2
  • Jean Boyer
    • 3
  • David B. Weiner
    • 3
  • Stuart Lessin
    • 4
  • William V. Williams
    • 5
  1. 1.Rheumatology Division, Department of MedicineUniversity of Pennsylvania School of MedicinePhiladelphia
  2. 2.Apollon Inc.Malvern
  3. 3.Department of Pathology and Laboratory MedicineUniversity of Pennsylvania School of MedicinePhiladelphia
  4. 4.Department of DermatologyUniversity of Pennsylvania School of Medicine and The Philadelphia Veterans Affairs Medical CenterPhiladelphia
  5. 5.Smith Kline Beecham PharmaceuticalsPhiladelphia

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