In Vitro Promoter Analysis Using Nuclear Extracts and G-Free Cassette Vectors

  • Martin J. Tymms
Part of the Methods in Molecular Biology™ book series (MIMB, volume 130)


Nuclear extracts prepared by appropriate methods contain all of the components for transcription (1). Although nuclear extracts are not defined like transcription systems utilizing purified transcription components (2, 3, 4) the extracts can be used to study aspects of promoter regulation. If suitable nuclear extracts can be prepared that contain the necessary transcription factors in an active state, and suitable DNA templates containing regulator sequences are available, processes such as differential gene expression (5,6) and hormonal simulation of transcription can be reconstituted (7,8).


Nuclear Extract Packed Cell Volume Control Template Dounce Homogenizer Polypropylene Centrifuge Tube 
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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Martin J. Tymms
    • 1
  1. 1.National Vision Research Institute of AustraliaMelbourneAustralia

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