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Bioassays of Inductive Interactions in Amphibian Development

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Developmental Biology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 135))

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Abstract

Amphibian embryos provide excellent material for understanding the establishment of the vertebrate body plan during early development. Fertilized eggs are readily obtained by hormone-induced spawning, and their developmental rate can be adjusted by ambient temperature regulation. Eggs and early embryos are large enough in size for surgical manipulations, especially when compared with eggs of other vertebrates. Also, embryos and isolated embryonic tissues can be easily cultured for a minimum of several weeks in a simple salt solution containing antibiotics. These advantageous features of amphibian eggs and embryos have led to the discovery of the primary embryonic organizer, various inducing factors, and a variety of cellular events involved in inductive interactions.

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Author information

Authors and Affiliations

  1. CREST, Japan Science and Technology Corp., The University of Tokyo, Tokyo, Japan

    Takashi Ariizumi

  2. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan

    Kazuhiro Takano & Makoto Asashima

  3. Department of Biology, Indiana University, Bloomington, IN

    George M. Malacinski

Authors
  1. Takashi Ariizumi
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  2. Kazuhiro Takano
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  3. Makoto Asashima
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  4. George M. Malacinski
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Editor information

Editors and Affiliations

  1. Thomas Jefferson University, Philadelphia, PA

    Rocky S. Tuan

  2. University of Pennsylvania, Philadelphia, PA

    Cecilia W. Lo

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© 2000 Humana Press Inc., Totowa, NJ

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Ariizumi, T., Takano, K., Asashima, M., Malacinski, G.M. (2000). Bioassays of Inductive Interactions in Amphibian Development. In: Walker, J.M., Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols. Methods in Molecular Biology™, vol 135. Humana Press. https://doi.org/10.1385/1-59259-685-1:89

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  • DOI: https://doi.org/10.1385/1-59259-685-1:89

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-852-3

  • Online ISBN: 978-1-59259-685-0

  • eBook Packages: Springer Protocols

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