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Developmental Biology Protocols pp 447–462Cite as

Mosaic Analysis in Caenorhabditis elegans

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 135))

Abstract

The complete description of its nearly invariant cell lineage (1,2) and the growing availability of cloned genes and markers for the cell lineage make Caenorhabditis elegans particularly favorable for mosaic analysis, and the literature is rich in examples that prove the usefulness of this approach. Because genetic mosaic analysis in C. elegans has recently been reviewed by Herman (3) who developed many of the techniques (3–6), this review will be more concerned with recent technical advances rather than with an extensive background of the approach. First we shall present a brief summary of the principles of mosaic analysis as it is typically performed in C. elegans. This will be followed by a description of markers that indicate mosaicism and by a discussion of a hypothetical analysis of an essential gene.

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Author information

Authors and Affiliations

  1. Department of Genetics and Cell Biology, University of Minnesota, St. Paul, MN

    John Yochem

  2. Department of Genetics, School of Medicine, University of Pennsylvania, Philadelphia, PA

    Meera Sundaram

  3. Department of Cell and Developmental Biology, School of Medicine, University of Pennsylvania, Philadelphia, PA

    Elizabeth A. Bucher

Authors
  1. John Yochem
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  2. Meera Sundaram
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  3. Elizabeth A. Bucher
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Editor information

Editors and Affiliations

  1. Thomas Jefferson University, Philadelphia, PA

    Rocky S. Tuan

  2. University of Pennsylvania, Philadelphia, PA

    Cecilia W. Lo

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© 2000 Humana Press Inc., Totowa, NJ

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Yochem, J., Sundaram, M., Bucher, E.A. (2000). Mosaic Analysis in Caenorhabditis elegans . In: Walker, J.M., Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols. Methods in Molecular Biology™, vol 135. Humana Press. https://doi.org/10.1385/1-59259-685-1:447

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  • DOI: https://doi.org/10.1385/1-59259-685-1:447

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-852-3

  • Online ISBN: 978-1-59259-685-0

  • eBook Packages: Springer Protocols

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