Abstract
Optical coherence tomography (OCT) is an attractive imaging technique for developmental biology because it permits the imaging of tissue microstructure in situ, yielding micron-scale image resolution without the need for excision of a specimen and tissue processing. OCT enables repeated imaging studies to be performed on the same specimen in order to track developmental changes. OCT is analogous to ultrasound B mode imaging except that it uses low-coherence light rather than sound and performs cross-sectional imaging by measuring the backscattered intensity of light from structures in tissue (1). The principles of OCT imaging are shown schematically in Fig. 1. The OCT image is a gray-scale or false-color two-dimensional (2-D) representation of backscattered light intensity in a cross-sectional plane. The OCT image represents the differential backscattering contrast between different tissue types on a micron scale. Because OCT performs imaging using light, it has a one- to two-order-of-magnitude higher spatial resolution than ultrasound and does not require specimen contact.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Huang, D., Swanson, E. A., Lin, C. P., Schuman, J. S., Stinson, W. G., Chang, W., et al. (1991) Optical coherence tomography. Science 254, 1178–1181.
Hee, M. R., Izatt, J. A., Swanson, E. A., Huang, D., Lin, C. P., Schuman, J. S., et al. (1995) Optical coherence tomography of the human retina. Arch. Ophthalmol. 113, 325–332.
Puliafito, C. A., Hee, M. R., Lin, C. P., Reichel, E., Schuman, J. S., Duker, J. S., et al. (1995) Imaging of macular disease with optical coherence tomography (OCT). Ophthalmology 102, 217–229.
Puliafito, C. A., Hee, M. R., Schuman, J. S., and Fujimoto, J. G. (1995) Optical Coherence Tomography of Ocular Diseases. Slack, Thorofare, NJ.
Schmitt, J. M., Knuttel, A., and Bonner, R. F. (1993). Measurement of the optical properties of biological tissue using low-coherence reflectometry. Appl. Opt. 32, 6032–6042.
Schmitt, J. M., Knuttel, A., Yadlowsky, M., and Eckhaus, A. A. (1994) Optical coherence tomography of a dense tissue: Statistics of attenuation and backscattering. Phys. Med. Biol. 39, 1705–1720.
Fujimoto, J. G., Brezinski, M. E., Tearney, G. J., Boppart, S. A., Bouma, B. E., et al. (1995) Biomedical imaging and optical biopsy using optical coherence tomography. Nat. Med. 1, 970–972.
Schmitt, J. M., Yadlowsky, M. J., and Bonner, R. F. (1995) Subsurface imaging of living skin with optical coherence microscopy. Dermatology 191, 93–98.
Sergeev, A., Gelikonov, B., Gelikonov, G., Feldchetin, F., Pravdenki, K., Kuranov, R., et al. (1995) High-spatial resolution optical-coherence tomography of human skin and mucus membranes in Conference on Lasers and Electro Optics ‘95, Vol. 15 of 1995 OSA Technical Digest Series (Optical Society of America, Washington, DC), paper CThN4.
Brezinski, M. E., Tearney, G. J., Bouma, B. E., Izatt, J. A., Hee, M. R., Swanson, E. A., et al. (1996) Optical coherence tomography for optical biopsy: Properties and demonstration of vascular pathology. Circulation 93, 1206–1213.
Tearney, G. J., Brezinski, M. E., Boppart, S. A., Bouma, B. E., Weissman, N., Southern, J. F., et al. (1996) Catheter-based optical imaging of a human coronary artery. Circulation 94, 3013.
Brezinski, M. E., Tearney, G. J., Weissman, N. J., Boppart, S. A., Bouma, B. E., Hee, M. R., et al. (1997) Assessing atherosclerotic plaque morphology: Comparison of optical coherence tomography and high frequency intravascular ultrasound. Br. Heart J. 77, 397–404.
Brezinski, M. E., Tearney, G. J., Boppart, S. A., Swanson, E. A., Southern, J. F., and Fujimoto, J. G. (1997) Optical biopsy with optical coherence tomography, feasibility for surgical diagnostics. J. Surg. Res. 71, 32–40.
Tearney, G. J., Brezinski, M. E., Southern, J. F., Bouma, B. E., Boppart, S. A., and Fujimoto, J. G. (1997) Optical biopsy in human gastrointestinal tissue using optical coherence tomography. Am. J. Gastroenterol. 92, 1800–1804.
Tearney, G. J., Brezinski, M. E., Southern, J. F., Bouma, B. E., Boppart, S. A., and Fujimoto, J. G. (1997) Optical biopsy in human urologic tissue using optical coherence tomography. J. Urol. 157, 1915–1919.
Bouma, B., Tearney, G. J., Boppart, S. A., Hee, M. R., Brezinski, M. E., and Fujimoto, J. G. (1995) High-resolution optical coherence tomographic imaging using a mode-locked Ti∶Al2O3 laser source. Opt. Lett. 20, 1486–1489.
Bouma, B. E., Tearney, G. J., Biliinski, I. P., Golubovic, B., and Fujimoto, J. G. (1996) Self-phase-modulated Kerr-lens mode-locked Cr:forsterite laser source for optical coherence tomography. Opt. Lett. 21, 1839–1842.
Tearney, G. J., Bouma, B. E., Boppart, B. E., Golubovic, B., Swanson, E. A., and Fujimoto, J. G. (1996) Rapid acquisition of in vivo biological images using optical coherence tomography. Opt. Lett. 21, 1408–1410.
Tearney, G. J., Bouma, B. E., and Fujimoto, J. G. (1997) High-speed phase-and group-delay scanning with a grating-based phase control delay line. Opt. Lett. 22, 1811–1813.
de Boer, J. F., Milner, T. E., van Germert, M. J. C., and Stuart Nelson, J. (1997) Two dimensional birefringence imaging in biological tissue by polarization sensitive optical coherence tomography. Opt. Lett. 22, 934–936.
Chen, Z., Milner, T. E., Srinivas, S., Wang, X., Malekafzali, A., van Germert, M. J. C., et al. (1997) Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography. Opt. Lett. 22, 1119–1120.
Izatt, J. A., Kulkarni, M. D., Yazdanfar, S., Barton, J. K., and Welch, A. J. (1997) In vivo bidirectional color doppler flow imaging of picoliter blood volumes using optical coherence tomography. Opt. Lett. 22, 1439–1441.
Tearney, G. J., Boppart, S. A., Bouma, B. E., Brezinski, M. E., Weissman, N. J., Southern, J. F., et al. (1996) Scanning single-mode fiber optic catheter-endoscope for optical coherence tomography. Opt. Lett. 21, 543–545.
Boppart, S. A., Bouma, B. E., Pitris, C., Tearney, G. J., Fujimoto, J. G., and Brezinski, M. E. (1997) Forward-scanning instruments for optical coherence tomographic imaging. Opt. Lett. 22, 1618–1620.
Tearney, G. J., Brezinski, M. E., Bouma, B. E., Boppart, S. A., Pitris, C., Southern, J. F., et al. (1997) In vivo endoscopic optical biopsy with optical coherence tomography. Science 276, 2037–2039.
Takada, K., Yokohama, I., Chida, K., and Noda, J. (1987) New measurement system for fault location in optical waveguide devices based on an interferometric technique. Appl. Opt. 26, 1603–1606.
Fercher, A. F., Mengedoht, K., and Werner, W. (1988) Eye-length measurement by interferometry with partially coherent light. Opt. Lett. 13, 186–190.
Hitzenberger, C. K. (1991) Measurement of the axial eye length by laser Doppler interferometry. Invest. Ophthalmol. Vis. Sci. 32, 616–624.
Chernikov, S. V., Zhu, Y., Taylor, J. R., Platonov, N. S., Samartsev, I. E., and Gapontsev, V. P. (1996) 1.08–2.2 μm supercontinuum generation from Yb3+ doped fiber laser. Conference on Lasers and Electro Optics CLEO 96, Vol. 9 of 1996 OSA Technical Digest Series (Optical Society of America, Washington, DC) paper CTuU4.
Swanson, E. A., Chinn, S. R., Hodgson, C. W., Bouma, B. E., Tearney, G. J., and Fujimoto, J. G. (1996) Spectrally shaped rare-earth doped fiber ASE sources for use in optical coherence tomography. Conference on Lasers and Electro Optics CLEO 96, Vol. 9 of OSA 1996 Technical Digest, (Optical Society of America, Washington, DC) paper CTuU5.
Chernikov, V., Taylor, J. R., Gapontsev, V. P., Bouma, B. E., and Fujimoto, J. G. (1997) A 75 nm, 30 mW superfluorescent ytterbium fiber source operation around 1.06 μm. Conference on Lasers and Electro Optics CLEO 97, Vol. 11 of OSA 1997 Technical Digest, (Optical Society of America, Washington, DC) paper CTuG8.
Bouma, B. E., Nelson, L. E., Tearney, G. J., Jones, D. J., Brezinski, M. E., and Fujimoto, J. G. (1998) Optical coherence tomographic imaging of human tissue at 1.55 μm and 1.8 μm using Er-and Tm-doped fiber sources. J. Biomed. Opt. 3, 76–79.
Boppart, S. A., Brezinski, M. E., Bouma, B. E., Tearney, G. J., and Fujimoto, J. G. (1996) Investigation of developing embryonic morphology using optical coherence tomography. Dev. Biol. 177, 54–63.
Boppart, S. A., Brezinski, M. E., Tearney, G. J., Bouma, B. E., and Fujimoto, J. G. (1996) Imaging developing neural morphology using optical coherence tomography. J. Neurosci. Methods 2112, 65–72.
Nieuwkoop, P. D. and Faber, J. (1994) Normal Table of Xenopus Laevis. Garland, New York.
Boppart, S. A., Tearney, G. J., Bouma, B. E., Southern, J. F., Brezinski, M. E., and Fujimoto, J. G. (1997) Noninvasive assessment of the developing xenopus cardiovascular system using optical coherence tomography. Proc. Natl. Acad. Sci. 94, 4256–4261.
Boppart, S. A., Bouma, B. E., Pitris, C., Southern, J. F., Brezinski, M. E., and Fujimoto, J. G. (1998) In vivo cellular optical coherence tomography imaging. Nat. Med. 4, 861–865.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Boppart, S.A., Brezinski, M.E., Fujimoto, J.G. (2000). Optical Coherence Tomography Imaging in Developmental Biology. In: Walker, J.M., Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols. Methods in Molecular Biology™, vol 135. Humana Press. https://doi.org/10.1385/1-59259-685-1:217
Download citation
DOI: https://doi.org/10.1385/1-59259-685-1:217
Publisher Name: Humana Press
Print ISBN: 978-0-89603-852-3
Online ISBN: 978-1-59259-685-0
eBook Packages: Springer Protocols