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Culture of Preimplantation Mouse Embryos

  • Adam S. Doherty
  • Richard M. Schultz
Part of the Methods in Molecular Biology™ book series (MIMB, volume 135)

Abstract

The preimplantation mammalian embryo develops as a free living entity within the mother. This internal development inherently precludes facile experimental manipulation necessary to study cellular and molecular mechanisms of preimplantation development. In turn, this has led to intense efforts over the course of decades to develop culture media that support the preimplantation development in vitro and, in particular, mouse preimplantation development. By the mid-1960s and early 1970s, these efforts led to the development of media such as Brinster’s modified oocyte culture (BMOC) (1) and Whitten’s medium (2). Further research examined the effect of the composition of the gas phase and led to the general conclusion that 5% oxygen was better than 21% oxygen, which is present in air. In addition, an empirically driven approach led to the formulation of culture media that supported development in vitro of one-cell embryos to the blastocyst stage (3,4) and overcame the two-cell block, which is exhibited following the culture of one-cell embryos obtained from outbred or inbred mice; embryos obtained from F1 hybrid mice do not exhibit the two-cell block.

Keywords

Blastocyst Stage Amino Acid Solution Preimplantation Development Preimplantation Mouse Embryo Mouse Preimplantation Development 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Brinster, R. L. (1965) Studies on the development of mouse embryos in vitro. IV. Interaction of energy sources. J. Reprod. Fertil. 10, 227–240.PubMedCrossRefGoogle Scholar
  2. 2.
    Whittne, W. K. (1971) Embryo medium. Nutrient requirements for the culture of pre-implantation embryo in vitro. Adv. Biosci. 6, 129–141.Google Scholar
  3. 3.
    Abramczuk, J., Solter, D., and Koprowski, H. (1977) The beneficial effect of EDTA on development of mouse one-cell embryos in chemically defined medium. Dev. Biol. 61, 378–383.PubMedCrossRefGoogle Scholar
  4. 4.
    Gardner, D. K. and Lane, M. (1996) Alleviation of the “2-cell block” and development to the blastocyst stage of CF1 mouse embryos: Role of amino acids, EDTA and physical parameters. Hum. Biol. 11, 2703–2712.Google Scholar
  5. 5.
    Biggers, J. D., Lawitts, J. A., and Lechene, C. P. (1992) The protective action of betaine on the deleterious effects of NaCl on preimplantation mouse embryos in vitro. Mol. Reprod. Dev. 34, 380–390.CrossRefGoogle Scholar
  6. 6.
    Lawitts, J. A. and Biggers, J. D. (1992) Joint effects of sodium chloride, glutamine, and glucose in mouse preimplantation embryo culture media. Mol. Reprod. Dev. 31, 189–194.PubMedCrossRefGoogle Scholar
  7. 7.
    Erbach, G. T., Lawitts, J. A., Papaioannou, V. E., and Biggers, J. D. (1994) Differential growth of the mouse preimplantation embryo in chemically defined media. Biol. Reprod. 50, 1027–1033.PubMedCrossRefGoogle Scholar
  8. 8.
    Summers, J. C., Bhatnagar, P. R., Lawitts, J. A., and Biggers, J. D. (1995) Fertilization in vitro of mouse ova from inbred and outbred stains: Complete preimplantation embryo development in glucose-supplemented KSOM. Biol. Reprod. 53, 431–437.PubMedCrossRefGoogle Scholar
  9. 9.
    Ho, Y., Wigglesworth, K., Eppig, J. J., and Schultz, R. M. (1995) Preimplantation development of mouse embryos in KSOM: Augmentation by amino acids and analysis of gene expression. Mol. Reprod. Dev. 41, 232–238.PubMedCrossRefGoogle Scholar
  10. 10.
    Baltz, J. M., Biggers, J. D., and Lechene, C. (1993) A novel H+ permeability dominating intracellular pH in the early mouse embryo. Development 118, 1353–1361.PubMedGoogle Scholar
  11. 11.
    Brison, D. R. and Schultz, R. M. (1997) Apoptosis during preimplantation mouse embryo formation: Evidence for a role for survival factors including TGF-α. Biol. Reprod. 56, 1088–1096.PubMedCrossRefGoogle Scholar
  12. 12.
    Erbach, G. T., Bhatnagar, P., Baltz, J. M., and Biggers, J. D. (1995) Zinc is a possible toxic contaminant of silicone oil in microdrop cultures of preimplantation mouse embryos. Hum. Reprod. 10, 3248–3254.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Adam S. Doherty
    • 1
  • Richard M. Schultz
    • 1
  1. 1.Department of BiologyUniversity of PennsylvaniaPhiladelphia

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