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Immunocytochemistry of NMDA Receptors

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NMDA Receptor Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 128))

Abstract

This chapter covers the major immunocytochemical methods used in our laboratory for NMDA receptor localization. There are three methods: pre-embedding immunoperoxidase for light microscopy (LM), the same for electron microscopy (EM), and postembedding immunogold (1–5). General versions of these techniques, as well as related techniques (immunofluorescence, immunocytochemistry of cultures) have been discussed and compared for immunolabeling of all types of glutamate receptors in Petralia and Wenthold (6). Also, for more information on interpretation of immunolabeling data for glutamate receptors, see Petralia and Wenthold (7) and Petralia (8). Various modifications of these methods have been described briefly (9–17).

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Author information

Authors and Affiliations

  1. Laboratory of Neurochemistry, National Institutes of Health, Bethesda, MD

    Ronald S. Petralia

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  1. Ronald S. Petralia
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  2. Robert J. Wenthold
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Editors and Affiliations

  1. Johns Hopkins School of Medicine, Baltimore, MD

    Min Li

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Petralia, R.S., Wenthold, R.J. (1999). Immunocytochemistry of NMDA Receptors. In: Li, M. (eds) NMDA Receptor Protocols. Methods in Molecular Biology™, vol 128. Humana Press. https://doi.org/10.1385/1-59259-683-5:73

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  • DOI: https://doi.org/10.1385/1-59259-683-5:73

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-713-7

  • Online ISBN: 978-1-59259-683-6

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