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Stable Expression of Human NMDA Receptors in Cultured Mammalian Cells

  • Mark A. Varney
  • Fen-Fen Lin
  • Christine Jachec
  • Sara P. Rao
  • Stephen D. Hess
  • Edwin C. Johnson
  • Gönül Veliçelebi
Part of the Methods in Molecular Biology™ book series (MIMB, volume 128)

Abstract

NMDA receptors are ligand-gated cation channels that are activated by glutamate and glycine. They are distributed throughout the mammalian CNS, and play an important role in normal development and plasticity of the CNS, and likely in such specialized functions as memory and learning. Prolonged activation of these channels has been implicated in several pathophysiological conditions, such as stroke and epilepsy. Therefore, NMDA receptors represent relevant molecular targets for therapeutic agents in the treatment of stroke, epilepsy, head trauma, and pain (1, 2, 3).

Keywords

NMDA Receptor HEK293 Cell G418 Sulfate Dialyze Fetal Bovine Serum Competition Binding Experiment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Mark A. Varney
    • 1
  • Fen-Fen Lin
    • 1
  • Christine Jachec
    • 1
  • Sara P. Rao
    • 1
  • Stephen D. Hess
    • 1
  • Edwin C. Johnson
    • 1
  • Gönül Veliçelebi
    • 2
  1. 1.SIBIA Neurosciences Inc.La Jolla
  2. 2.MitoKorSan Diego

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