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Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes

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Book cover T Cell Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 134))

Abstract

CD45 is a leukocyte specific protein tyrosine phosphatase and is a major lymphocyte cell surface glycoprotein (see refs. 1–3 for reviews). CD45 has been shown to be important for the transmission of signals by the T and B cell antigen receptors during both development and antigen induced activation. The phosphatase activity of CD45 has been shown to be required for efficient signaling by the T-cell receptor complex, yet little is known of how or whether the phosphatase activity of CD45 is regulated. In this chapter, we describe a simple and sensitive method to measure the phosphatase activity of CD45 isolated directly from T cells.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada

    David H. W. Ng & Jackie Felberg

Authors
  1. David H. W. Ng
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  2. Jackie Felberg
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  3. Pauline Johnson
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Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC

    Kelly P. Kearse

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© 2000 Humana Press Inc., Totowa, NJ

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Ng, D.H.W., Felberg, J., Johnson, P. (2000). Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes. In: Kearse, K.P. (eds) T Cell Protocols. Methods in Molecular Biology™, vol 134. Humana Press. https://doi.org/10.1385/1-59259-682-7:289

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  • DOI: https://doi.org/10.1385/1-59259-682-7:289

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-810-3

  • Online ISBN: 978-1-59259-682-9

  • eBook Packages: Springer Protocols

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