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Chromatin Protocols pp 497–508Cite as

An In Vivo UV Crosslinking Assay That Detects DNA Binding by Sequence-Specific Transcription Factors

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 119))

Abstract

In vivo UV crosslinking permits direct analysis of protein-DNA interactions in intact cells. This technique has been used to study DNA binding by a wide variety of proteins including RNA Polymerase II, Topoisomerase I, and sequence specific transcription factors such as Even-Skipped, Zeste, and GAGA (1–4). For many of these proteins, the pattern of DNA binding discovered by UV crosslinking differs dramatically from that predicted by earlier indirect approaches. This has led to fundamental reassessments of how these proteins act and illustrates the importance of examining protein-DNA interactions in vivo.

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Author information

Authors and Affiliations

  1. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT

    Alan Carr & Mark D. Biggin

Authors
  1. Alan Carr
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  2. Mark D. Biggin
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Editor information

Editors and Affiliations

  1. Adolf-Butenandt-Institut-Molekularbiologie, Ludwig-Maximilians-Universität, München, Germany

    Peter B. Becker

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© 1999 Humana Press Inc.

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Carr, A., Biggin, M.D. (1999). An In Vivo UV Crosslinking Assay That Detects DNA Binding by Sequence-Specific Transcription Factors. In: Becker, P.B. (eds) Chromatin Protocols. Methods in Molecular Biology™, vol 119. Humana Press. https://doi.org/10.1385/1-59259-681-9:497

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  • DOI: https://doi.org/10.1385/1-59259-681-9:497

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-665-9

  • Online ISBN: 978-1-59259-681-2

  • eBook Packages: Springer Protocols

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