Abstract
Histones are the predominant protein component of chromatin and are subject to a variety of post-translational modifications. Of these, acetylation of the amino-terminal tails of core histones is most intensively studied and is linked to chromatin assembly, the regulation of gene expression, cell cycle progression and cellular transformation (1). Characterization of the enzymes responsible for histone acetylation provides a handle for directly studying such histone modifications in these processes. Two classes of histone acetyltransferases (HATs) have been described; cytoplasmic type B HATs acetylate free histones for subsequent assembly into chromatin (2) and nuclear type A HATs, which mediate transcription related acetylation of chromosomal histones (3).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Wade, P. A., Pruss, D., and Wolffe, A. (1997) Histone acetylation: chromatin in action. Trends Biochem. 22, 128–132.
Roth, S. Y. and Allis, C. D. (1996) Histone acetylation and chromatin assembly: A single escort, multiple dances? Cell 87, 5–8.
Brownell, J. E. and Allis, C. D. (1996) Special HATs for special occasions: Linking histone acetylation to chromatin assembly and gene activation. Curr. Opin. Genet. Develop. 6, 176–184.
Brownell, J. E., Zhou, J., Ranalli, T., Kobayashi, R., Edmondson, D. G., Roth, S. Y., and Allis, C. D. (1996) Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation. Cell 84, 843–851.
Grant, P. A., Duggan, L., Côté, J., Roberts, S. M., Brownell, J., Candau, R., Ohba, R., Owen-Hughes, T., Allis, C. D., Winston, F., Berger, S. L., and Workman, J. L. (1997) Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: Characterization of an ADA and the SAGA (Spt/Ada) complex. Genes Dev. 11, 1640–1650.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc.
About this protocol
Cite this protocol
Grant, P.A., Berger, S.L., Workman, J.L. (1999). Identification and Analysis of Native Nucleosomal Histone Acetyltransferase Complexes. In: Becker, P.B. (eds) Chromatin Protocols. Methods in Molecular Biology™, vol 119. Humana Press. https://doi.org/10.1385/1-59259-681-9:311
Download citation
DOI: https://doi.org/10.1385/1-59259-681-9:311
Publisher Name: Humana Press
Print ISBN: 978-0-89603-665-9
Online ISBN: 978-1-59259-681-2
eBook Packages: Springer Protocols