An In Vivo UV Crosslinking Assay That Detects DNA Binding by Sequence-Specific Transcription Factors

  • Alan Carr
  • Mark D. Biggin
Part of the Methods in Molecular Biology™ book series (MIMB, volume 119)


In vivo UV crosslinking permits direct analysis of protein-DNA interactions in intact cells. This technique has been used to study DNA binding by a wide variety of proteins including RNA Polymerase II, Topoisomerase I, and sequence specific transcription factors such as Even-Skipped, Zeste, and GAGA (1, 2, 3, 4). For many of these proteins, the pattern of DNA binding discovered by UV crosslinking differs dramatically from that predicted by earlier indirect approaches. This has led to fundamental reassessments of how these proteins act and illustrates the importance of examining protein-DNA interactions in vivo.


Wash Solution Tissue Culture Cell Full Speed CsCl Gradient High Background Signal 
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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Alan Carr
    • 1
  • Mark D. Biggin
    • 1
  1. 1.Department of Molecular Biophysics and BiochemistryYale UniversityNew Haven

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