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Molecular Diagnosis of Infectious Diseases pp 91–106Cite as

Expression Cloning

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 94))

Abstract

Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects. Here we describe the detailed construction of a genomic, random shear lambda expression library, adsorption of anti Escherichia coli antibody from antiserum, the screening of an expression library with specific antisera, and the cloning of genes with potential use in the diagnosis of infectious disease. This approach has been used successfully by our laboratory for the discovery of antigenic components of diagnostics and vaccines for several infectious agents including: Mycobacterium tuberculosis, Anaplasma phagocytophila (formerly Ehrlichia spp. or E. phagocytophila), Babesia microti, Trypanosoma cruzi, Leishmania chagasi, and Chlamydia spp.

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Author information

Authors and Affiliations

  1. Corixa Corporation, Seattle, WA

    Michael J. Lodes, Davin C. Dillon, Raymond L. Houghton & Yasir A. W. Skeiky

Authors
  1. Michael J. Lodes
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  2. Davin C. Dillon
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  3. Raymond L. Houghton
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  4. Yasir A. W. Skeiky
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Editor information

Editors and Affiliations

  1. Accenture GmbH, Munich, Germany

    Jochen Decler

  2. Institute for Medical Microbiology and Hygiene, University of Regensburg, Regenburg, Germany

    Udo Reischl

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© 2004 Humana Press Inc.

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Lodes, M.J., Dillon, D.C., Houghton, R.L., Skeiky, Y.A.W. (2004). Expression Cloning. In: Decler, J., Reischl, U. (eds) Molecular Diagnosis of Infectious Diseases. Methods in Molecular Medicine™, vol 94. Humana Press. https://doi.org/10.1385/1-59259-679-7:91

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  • DOI: https://doi.org/10.1385/1-59259-679-7:91

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-221-6

  • Online ISBN: 978-1-59259-679-9

  • eBook Packages: Springer Protocols

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