Skip to main content
SpringerLink
Log in

Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors

  • Protocol
Molecular Diagnosis of Infectious Diseases

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 94))

Abstract

The use of biosensors employing surface plasmon resonance (SPR) provides excellent instrumentation for a label-free, real-time investigation of biomolecular interactions. A broad range of biological applications including antibody-antigen interactions can be analyzed. One major advantage of kinetic analysis using SPR-based biosensors is the option of determining separately distinct association and dissociation rate constants exceeding the classical steady-state analysis of biomolecules. Based on these data new possibilities for drug design, characterizing human pathogens, and the development of therapeutic antibodies can be achieved. The hardware of commercially available systems is described, practical step by step procedures are given, and possibilities and limitations of the technology are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Rich, R. L. and Myszka, D. G. (2001) Survey of the year 2000 commercial optical biosensor literature. J. Mol. Recognit. 14, 273–294s.

    Article  PubMed  CAS  Google Scholar 

  2. Wink, T., de Beer, J., Hennink, W. E., Bult, A., and van Bennekom, W. P. (1999) Interaction between plasmid DNA and cationic polymers studied by surface plasmon resonance spectrometry. Anal. Chem. 71, 801–805.

    Article  CAS  Google Scholar 

  3. Baird, C. L. and Myszka, D. G. (2001) Current and emerging commercial optical biosensors. J. Mol. Recognit. 14, 261–268.

    Article  PubMed  CAS  Google Scholar 

  4. Stenberg, E., Persson, B., Roos, H., and Urbaniczky, C. (1991) Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins. J. Colloid Interface Sci. 143, 513–526.

    Article  CAS  Google Scholar 

  5. Kovacs, G. D. (1982) In Electromagnetic Surface Modes (Boardman, A. D., ed.), Wiley, New York, p. 143.

    Google Scholar 

  6. Zimmermann, B., Hahnefeld, C., and Herberg, F. W. (2002) Applications of biomolecular interaction analysis in drug development. TARGETS 1, 66–73.

    Article  CAS  Google Scholar 

  7. Jokiranta, T. S., Westin, J., Nilsson, U. R., et al. (2001) Complement C3b interactions studied with surface plasmon resonance technique. Int. Immunopharmacol. 1, 495–506s.

    Article  PubMed  CAS  Google Scholar 

  8. Jokiranta, T. S., Hellwage, J., Koistinen, V., Zipfel, P. F., and Meri, S. (2000) Each of the three binding sites on complement factor H interacts with a distinct site on C3b. J. Biol. Chem. 275, 27,657–27,662.

    PubMed  CAS  Google Scholar 

  9. Jarva, H., Janulczyk, R., Hellwage, J., Zipfel, P. F., Bjorck, L., and Meri, S. (2002) Streptococcus pneumoniae evades complement attack and opsonophagocytosis by expressing the pspC locus-encoded Hic protein that binds to short consensus repeats 8-11 of factor H. J. Immunol. 168, 1886–1894.

    PubMed  CAS  Google Scholar 

  10. Hellwage, J., Meri, T., Heikkila, T., et al. (2001) The complement regulator factor H binds to the surface protein OspE of Borrelia burgdorferi. J. Biol. Chem. 276, 8427–8435.

    Article  PubMed  CAS  Google Scholar 

  11. Meri, T., Hartmann, A., Lenk, D., et al. (2002) The yeast Candida albicans binds complement regulators factor H and FHL-1. Infect. Immun. 70, 5185–5192.

    Article  PubMed  CAS  Google Scholar 

  12. Sidobre, S., Puzo, G., and Riviere, M. (2002) Lipid-restricted recognition of mycobacterial lipoglycans by human pulmonary surfactant protein A: a surface-plasmon-resonance study. Biochem. J. 365, 89–97.

    Article  PubMed  CAS  Google Scholar 

  13. Jongerius-Gortemaker, B. G., Goverde, R. L., van Knapen, F., and Bergwerff, A. A. (2002) Surface plasmon resonance (BIACORE) detection of serum antibodies against Salmonella enteritidis and Salmonella typhimurium. J. Immunol. Methods 266, 33–44.

    Article  PubMed  CAS  Google Scholar 

  14. Hifumi, E., Kubota, N., Niimi, Y., Shimizu, K., Egashira, N., and Uda, T. (2002) Elimination of ingredients effect to improve the detection of anti HIV-1 p24 antibody in human serum using SPR apparatus. Anal. Sci. 18, 863–867.

    Article  PubMed  CAS  Google Scholar 

  15. McLaurin, J., Cecal, R., Kierstead, M. E., et al. (2002) Therapeutically effective antibodies against amyloid-beta peptide target amyloid-beta residues 4–10 and inhibit cytotoxicity and fibrillogenesis. Nat. Med. 8, 1263–1269.

    Article  PubMed  CAS  Google Scholar 

  16. Gonzales, N. R., Schuck, P., Schlom, J., and Kashmiri, S. V. (2002) Surface plasmon resonance-based competition assay to assess the sera reactivity of variants of humanized antibodies. J. Immunol. Methods 268, 197–210.

    Article  PubMed  CAS  Google Scholar 

  17. Alaedini, A. and Latov, N. (2001) A surface plasmon resonance biosensor assay for measurement of anti-GM(1) antibodies in neuropathy. Neurology 56, 855–860.

    PubMed  CAS  Google Scholar 

  18. Herberg, F. W. and Zimmermann, B. (1999) Analysis of protein kinase interactions using biomolecular interaction analysis, in Protein Phosphorylation—A Practical Approach (Hardie, D. G., ed.), vol. 2, Oxford University Press, Oxford, pp. 335–371.

    Google Scholar 

  19. Myszka, D. G. (2000) Kinetic, equilibrium, and thermodynamic analysis of macromolecular interactions with BIACORE. Methods Enzymol. 323, 325–340.

    Article  PubMed  CAS  Google Scholar 

  20. Karlsson, R. and Falt, A. (1997) Experimental design for kinetic analysis of protein-protein interactions with surface plasmon resonance biosensors. J. Immunol. Methods 200, 121–133.

    Article  PubMed  CAS  Google Scholar 

  21. Hall, D. R., Gorgani, N. N., Altin, J. G., and Winzor, D. J. (1997) Theoretical and experimental considerations of the pseudo-first-order approximation in conventional kinetic analysis of IAsys biosensor data. Anal. Biochem. 253, 145–155.

    Article  PubMed  CAS  Google Scholar 

  22. O’Shannessy, D. J. and Winzor, D. J. (1996) Interpretation of deviations from pseudo-first-order kinetic behavior in the characterization of ligand binding by biosensor technology. Anal. Biochem. 236, 275–283.

    Article  Google Scholar 

  23. Hall, D. R., Cann, J. R., and Winzor, D. J. (1996) Demonstration of an upper limit to the range of association rate constants amenable to study by biosensor technology based on surface plasmon resonance. Anal. Biochem. 235, 175–184.

    Article  PubMed  CAS  Google Scholar 

  24. Schuck, P. and Minton, A. P. (1996) Analysis of mass transport-limited binding kinetics in evanescent wave biosensors. Anal. Biochem. 240, 262–272.

    Article  PubMed  CAS  Google Scholar 

  25. Natsume, T., Nakayama, H., and Isobe, T. (2001) BIA-MS-MS: biomolecular interaction analysis for functional proteomics. Trends Biotechnol. 19(10 suppl), S28–33.

    Article  PubMed  CAS  Google Scholar 

  26. Cain, K. D., Jones, D. R., and Raison, R. L. (2002) Antibody-antigen kinetics following immunization of rainbow trout (Oncorhynchus mykiss) with a T-cell dependent antigen. Dev. Comp. Immunol. 26, 181–190.

    Article  PubMed  CAS  Google Scholar 

  27. Ciolkowski, M. L., Fang, M. M., and Lund, M. E. (2000) A surface plasmon resonance method for detecting multiple modes of DNA-ligand interactions. J. Pharm. Biomed. Anal. 22, 1037–1045.

    Article  PubMed  CAS  Google Scholar 

  28. Schindler, J. F., Godbey, A., Hood, W. F., et al. (2002) Examination of the kinetic mechanism of mitogen-activated protein kinase activated protein kinase-2. Biochim. Biophys. Acta 1598, 88–97.

    PubMed  CAS  Google Scholar 

  29. Hoffman, T. L., Canziani, G., Jia, L., Rucker, J., and Doms, R. W. (2000) A biosensor assay for studying ligand-membrane receptor interactions: binding of antibodies and HIV-1 Env to chemokine receptors. Proc. Natl. Acad. Sci. USA 97, 11,215–11,220.

    Article  PubMed  CAS  Google Scholar 

  30. Hwang, J., Fauzi, H., Fukuda, K., et al. (2000) The RNA aptamer-binding site of hepatitis C virus NS3 protease. Biochem. Biophys. Res. Commun. 279, 557–562.

    Article  PubMed  CAS  Google Scholar 

  31. Kamionka, A. and Dahl, M. K. (2001) Bacillus subtilis contains a cyclodextrin-binding protein which is part of a putative ABC-transporter. FEMS Microbiol. Lett. 204, 55–60.

    Article  PubMed  CAS  Google Scholar 

  32. Baird, C. L., Courtenay, E. S., and Myszka, D. G. (2002) Surface plasmon resonance characterization of drug/liposome interactions. Anal. Biochem. 310, 93–99.

    Article  PubMed  CAS  Google Scholar 

  33. Satoh, A., Hazuki, M., Kojima, K., Hirabayashi, J., and Matsumoto, I. (2000) Ligand-binding properties of annexin from Caenorhabditis elegans (annexin XVI, Nex-1). J. Biochem. (Tokyo) 128, 377–381.

    CAS  Google Scholar 

  34. Schlecht, U., Nomura, Y., Bachmann, T., and Karube, I. (2002) Reversible surface thiol immobilization of carboxyl group containing haptens to a BIAcore biosensor chip enabling repeated usage of a single sensor surface. Bioconjug. Chem. 13, 188–193s.

    Article  PubMed  CAS  Google Scholar 

  35. Gau, J. J., Lan, E. H., Dunn, B., Ho, C. M., and Woo, J. C. (2001) A MEMS based amperometric detector for E. coli bacteria using self-assembled monolayers. Biosens. Bioelectron. 16, 745–755.

    Article  PubMed  CAS  Google Scholar 

  36. Cooper, M. A., Fiorini, M. T., Abell, C., and Williams, D. H. (2000) Binding of vancomycin group antibiotics to D-alanine and D-lactate presenting self-assembled monolayers. Bioorg. Med. Chem. 8, 2609–2616.

    Article  PubMed  CAS  Google Scholar 

  37. Langmuir, I. (1916) The constitution and fundamental properties of solids and liquids. Part I. Solids. J. Am. Chem. Soc. 38, 2221–2295.

    Article  CAS  Google Scholar 

  38. Langmuir, I. (1918) The adsorption of gases on plane surfaces of glass, mica and platinium. J. Am. Chem. Soc. 40, 1361–1404.

    Article  CAS  Google Scholar 

  39. Hänel, C. and Gauglitz, G. (2002) Comparison of reflectometric interference spectroscopy with other instruments for label-free optical detection. Anal. Bioanal. Chem. 372, 91–100.

    Article  PubMed  Google Scholar 

  40. Hengerer, A., Decker, J., Prohaska, E., Hauck, S., Kosslinger, C., and Wolf, H. (1999) Quartz crystal microbalance (QCM) as a device for the screening of phage libraries. Biosens. Bioelectron. 14, 139–144.

    Article  PubMed  CAS  Google Scholar 

  41. Matsuno, H., Niikura, K., and Okahata, Y. (2001) Direct monitoring kinetic studies of DNA polymerase reactions on a DNA-immobilized quartz-crystal microbalance. Chemistry 7, 3305–3312.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Kassel, FB 18, Kassel, Germany

    Claudia Hahnefeld & Friedrich W. Herberg

  2. Biaffin GmbH & Co KG, Kassel, Germany

    Stephan Drewianka

Authors
  1. Claudia Hahnefeld
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Stephan Drewianka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Friedrich W. Herberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Accenture GmbH, Munich, Germany

    Jochen Decler

  2. Institute for Medical Microbiology and Hygiene, University of Regensburg, Regenburg, Germany

    Udo Reischl

Rights and permissions

Reprints and permissions

Copyright information

© 2004 Humana Press Inc.

About this protocol

Cite this protocol

Hahnefeld, C., Drewianka, S., Herberg, F.W. (2004). Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors. In: Decler, J., Reischl, U. (eds) Molecular Diagnosis of Infectious Diseases. Methods in Molecular Medicine™, vol 94. Humana Press. https://doi.org/10.1385/1-59259-679-7:299

Download citation

  • DOI: https://doi.org/10.1385/1-59259-679-7:299

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-221-6

  • Online ISBN: 978-1-59259-679-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation