Abstract
The use of recombinant antigens is essential for the construction of robust and sensitive diagnostic assays. A critical step in the preparation of recombinant antigens is protein purification. Purification problems may be very different for related structural proteins expressed in the same host or for the same protein expressed in different hosts, because the biochemical characteristics of a recombinant protein, expressed in a heterologous system, are unique. In this chapter we make a brief introduction to protein purification procedures and we present a quick purification process suitable for the isolation of recombinant protein having high isoelectric points encoding non-conformational epitopes.
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Calogero, R.A., Aulicino, A. (2004). Purification of Recombinant Proteins with High Isoelectric Points. In: Decler, J., Reischl, U. (eds) Molecular Diagnosis of Infectious Diseases. Methods in Molecular Medicine™, vol 94. Humana Press. https://doi.org/10.1385/1-59259-679-7:225
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DOI: https://doi.org/10.1385/1-59259-679-7:225
Publisher Name: Humana Press
Print ISBN: 978-1-58829-221-6
Online ISBN: 978-1-59259-679-9
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