Abstract
Over the last 10 years, the animal cap of the Xenopus laevis embryo has proved to be a versatile test tissue for a variety of molecules involved not only in animal development but also vertebrate cell regulation in general. These molecules include growth factors (1–3), cell surface receptors (4–6), signal transduction molecules (7,8), transcription factors (9), and extracellular matrix molecules (10). The “animal cap assay” provides a simple, quick, inexpensive, and quantitative bioassay for biological activity of both cloned genes and purified or unpurified proteins.
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References
Kimelman, D. and Kirschner, M. (1987) Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo. Cell 51, 869–877.
Smith, J. C., Price, B. M., Van Nimmen, K., and Huylebroeck, D. (1990) Identification of a potent Xenopus mesoderm-inducing factor as a homologue of activin A. Nature 345, 729–731.
Hogan, B. L., Blessing, M., Winnier, G. E., Suzuki, N., and Jones, C. M. (1994) Growth factors in development: the role of TGF-beta related polypeptide signalling molecules in embryogenesis. Development 120, 53–60.
Amaya, E., Musci, T. J., and Kirschner, M. W. (1991) Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos. Cell 66, 257–270.
Hemmati-Brivanlou, A. and Melton, D. A. (1992) A truncated activin receptor inhibits mesoderm induction and formation of axial structures in Xenopus embryos. Nature 359, 609–614.
Howard, J. E., Hirst, E. M., and Smith, J. C. (1992) Are beta 1 integrins involved in Xenopus gastrulation? Mech. Devices 38, 109–119.
Graff, J. M., Bansal, A., and Melton, D. A. (1996) Xenopus Mad proteins transduce distinct subsets of signals for the TGF beta superfamily. Cell 85, 479–487.
Massague, J. (1996) TGFbeta signaling: receptors, transducers, and Mad proteins. Cell 85, 947–950.
Cunliffe, V. and Smith, J. C. (1992) Ectopic mesoderm formation in Xenopus embryos caused by widespread expression of a Brachyury homologue. Nature 358, 427–430.
Brickman, MC and Gerhart, JC (1994) Heparitinase inhibition of mesoderm induction and gastrulation in Xenopus laevis embryos. Dev. Biol. 164, 484–501.
Cooke, J., Smith, J. C., Smith, E. J., and Yaqoob, M. (1987) The organization of mesodermal pattern in Xenopus laevis: experiments using a Xenopus mesoderm-inducing factor. Development 101, 893–908.
Smith, J. C., Yaqoob, M., and Symes, K. (1988) Purification, partial characterization and biological effects of the XTC mesoderm-inducing factor. Development 103, 591–600.
Slack, J. M., Darlington, B. G., Heath, J. K., and Godsave, S. F. (1987) Mesoderm induction in early Xenopus embryos by heparin-binding growth factors. Nature 326, 197–200.
Smith, J. C. (1987) A mesoderm inducing factor is produced by a Xenopus cell line. Development 99, 3–14.
Green, J. B. A., Howes, G., Symes, K., Cooke, J., and Smith, J. C. (1990) The biological effects of XTC-MIF: quantitative comparison with Xenopus bFGF. Development 108, 173–183.
Dale, L., Howes, G., Price, B. M., and Smith, J. C. (1992) Bone morphogenetic protein 4, a ventralizing factor in early Xenopus development. Development 115, 573–585.
Graff, J. M., Thies, R. S., Song, J. J., Celeste, A. J., and Melton, D. A. (1994) Studies with a Xenopus BMP receptor suggest that ventral mesoderm-inducing signals override dorsal signals in vivo. Cell 79, 169–179.
Suzuki, A., Thies, R. S., Yamaji, N., Song, J. J., Wozney, J. M., Murakami, K., and Ueno, N. (1994) A truncated bone morphogenetic protein receptor affects dorsal-ventral patterning in the early Xenopus embryo. Proc. Natl. Acad. Sci. USA 91, 10,255–10,259.
Maeno, M., Ong, R. C., Suzuki, A., Ueno, N., and Kung, H. F. (1994) A truncated bone morphogenetic protein 4 receptor alters the fate of ventral mesoderm to dorsal mesoderm: roles of animal pole tissue in the development of ventral mesoderm. Proc. Natl. Acad. Sci. USA 91, 10,260–10,264.
Peng, H. B. and Kay, B. K. (eds.) (1991) Xenopus laevis: practical uses in cell and molecular biology, in Methods in Cell Biology, Academic, New York.
Green, J. B. A., New, H. V., and Smith, J. C. (1992) Responses of embryonic Xenopus cells to activin and FGF are separated by multiple dose thresholds and correspond to distinct axes of the mesoderm. Cell 71, 731–739.
Lustig, K. D., Kroll, K. L., Sun, E. E., and Kirschner, M. W. (1996) Expression cloning of a Xenopus T-related gene (Xombi) involved in mesodermal patterning and blastopore lip formation. Development 122, 4001–4012.
Gurdon, J. B., Harger, P., Mitchell, A., and Lemaire, P. (1994) Activin signalling and response to a morphogen gradient. Nature 371, 487–492.
Gurdon, J. B., Mitchell, A., and Mahony, D. (1995) Direct and continuous assessment by cells of their position in a morphogen gradient. Nature 376, 520–521.
Kintner, C. R. and Dodd, J. (1991) Hensen’s node induces neural tissue in Xenopus ectoderm. Implications for the action of the organizer in neural induction. Development 113, 1495–1505.
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© 1999 Humana Press Inc.
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Green, J. (1999). The Animal Cap Assay. In: Guille, M. (eds) Molecular Methods in Developmental Biology. Methods in Molecular Biology™, vol 127. Humana Press. https://doi.org/10.1385/1-59259-678-9:1
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DOI: https://doi.org/10.1385/1-59259-678-9:1
Publisher Name: Humana Press
Print ISBN: 978-0-89603-790-8
Online ISBN: 978-1-59259-678-2
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