Immunohistochemistry of Xenopus Embryos

  • Carl Robinson
  • Matt Guille
Part of the Methods in Molecular Biology™ book series (MIMB, volume 127)

Abstract

Immunohistochemistry is a very powerful technique for determining both the tissue-specific and subcellular location of endogenous and exogenous proteins within an embryo. The technique is relatively simple and when used on its own or in conjunction with other immunological techniques, such as Western blotting, immunoblocking, and supershift assays (see  Chapter 15), can provide a large amount of information about the potential function or regulation of a given protein in a relatively small amount of time.

Keywords

Vortex H2O2 Glycerol Explosive HEPES 

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Carl Robinson
    • 1
  • Matt Guille
    • 1
  1. 1.Biophysics Laboratories, Institute of Biomolecular and Biomedical SciencesUniversity of PortsmouthPortsmouthUK

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