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Localization of HIV-1 DNA and Tumor Necrosis Factor-α mRNA in Human Brain Using Polymerase Chain Reaction In Situ Hybridization and Immunocytochemistry

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In Situ Hybridization Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 123))

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Abstract

We have had a long term interest in the neuropathogenesis of human immunodeficiency virus (HIV)-associated neurological diseases. Neurological disease is a prominent feature of HIV-1 infection, usually occurring during the late stages of acquired immunodeficiency syndrome (AIDS). infections and neoplasms in the brain arise as a consequence of severe immunosuppression, and HIV infection of the brain has been implicated in the pathogenesis of a progressive cognitive decline termed HIV-associated dementia. In light of the clinical evidence for neuronal dysfunction in HIV-related neurological disease and the pathological evidence for loss of neurons, a puzzling feature of brain infection with HIV is that the overwhelming productively infected cells are brain macrophages and microglia, and not cells of neuroectodermal origin such as neurons, astrocytes, and oligodendrocytes. This has been demonstrated repeatedly in many laboratories by the methods of immunocytochemistry for detection of HIV proteins and by in situ hybridization (ISH) for HIV RNA.

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Author information

Authors and Affiliations

  1. Alfred Infectious Disease Unit, Alfred Hospital, Prahran, Victoria, Australia

    Steven L. Wesselingh

  2. Department of Neurology, School of Medicine, Emory University, Atlanta, GA

    Jonathan D. Glass

Authors
  1. Steven L. Wesselingh
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  2. Jonathan D. Glass
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Editor information

Editors and Affiliations

  1. RMIT University Bundoora, Victoria, Australia

    Ian A. Darby

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© 2000 Humana Press Inc.

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Wesselingh, S.L., Glass, J.D. (2000). Localization of HIV-1 DNA and Tumor Necrosis Factor-α mRNA in Human Brain Using Polymerase Chain Reaction In Situ Hybridization and Immunocytochemistry. In: Darby, I.A. (eds) In Situ Hybridization Protocols. Methods in Molecular Biology™, vol 123. Humana Press. https://doi.org/10.1385/1-59259-677-0:323

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  • DOI: https://doi.org/10.1385/1-59259-677-0:323

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-686-4

  • Online ISBN: 978-1-59259-677-5

  • eBook Packages: Springer Protocols

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