Ultrastructural Detection of Nucleic Acids on Thin Sections of Tissue Embedded in Hydrophilic Resin

  • Dominique Le Guellec
Part of the Methods in Molecular Biology™ book series (MIMB, volume 123)


Detection of nucleic acid sequences at the ultrastructural level allows the localization of RNA and DNA molecules to specific subcellular compartments. At the light microscope level, it is already evident that DNA and RNA sequences do not have a random distribution, suggesting that their localization inside the cell is related to their role, or the role of their transcribed proteins.


Gold Particle Label Probe Hybridization Buffer Dextran Sulfate Nucleic Acid Sequence 
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Dominique Le Guellec
    • 1
  1. 1.Institut de Biologie et Chimie des Protéines (IBCP)LyonFrance

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