In Situ End-Labeling of Fragmented DNA and the Localization of Apoptosis

  • Tim D. Hewitson
  • Teresa Bisucci
  • Ian A. Darby
Part of the Methods in Molecular Biology™ book series (MIMB, volume 123)


Apoptosis is a morphologically distinct form of programmed cell death. It has a role in such processes as embryogenesis, immune regulation, and defense against viruses, and can also be induced by a variety of physical and chemical stimuli (1). Importantly apoptosis leads to the safe removal of cells by phagocytosis, whereas in contrast, necrosis provokes tissue injury and inflammation.


Terminal Deoxynucleotidyl Transferase Cobalt Chloride Primary Antiserum Protease Digestion TUNEL Label 
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Tim D. Hewitson
    • 1
  • Teresa Bisucci
    • 2
  • Ian A. Darby
    • 2
  1. 1.Department of NephrologyRoyal Melbourne HospitalAustralia
  2. 2.Wound Healing and Microvascular Biology Group, Department of Human Biology and Movement ScienceRMIT UniversityBundooraAustralia

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