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DNA-Protein In Situ Covisualization for Chromosome Analysis

  • Henry H. Q. Heng
  • Barbara Spyropoulos
  • Peter B. Moens
Part of the Methods in Molecular Biology™ book series (MIMB, volume 123)

Abstract

Although fluorescent in situ hybridization (FISH) technology has been used extensively for gene mapping and genome analysis (1-8), methods that visualize the interaction of DNA and protein are required to elucidate the functional aspects of the chromosome. By displaying the physical sites of molecular reactions in the highly organized compartments of the nucleus, this methodology provides more specific information on chromatin domain, chromosome, and nuclear position than data generated by other non-in situ DNA-protein interaction assays such as gel retardation analysis.

Keywords

Sodium Dodecyl Sulfate Wash Solution Chromosome Core Plastic Coverslip Antibody Dilution Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Henry H. Q. Heng
    • 1
  • Barbara Spyropoulos
    • 2
  • Peter B. Moens
    • 2
  1. 1.Center for Molecular Medicine and Genetics, School of MedicineWayne State UniversityDetroit
  2. 2.Department of BiologyYork UniversityDownsviewCanada

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