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DNA-Protein In Situ Covisualization for Chromosome Analysis

  • Protocol
In Situ Hybridization Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 123))

Abstract

Although fluorescent in situ hybridization (FISH) technology has been used extensively for gene mapping and genome analysis (1-8), methods that visualize the interaction of DNA and protein are required to elucidate the functional aspects of the chromosome. By displaying the physical sites of molecular reactions in the highly organized compartments of the nucleus, this methodology provides more specific information on chromatin domain, chromosome, and nuclear position than data generated by other non-in situ DNA-protein interaction assays such as gel retardation analysis.

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References

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Author information

Authors and Affiliations

  1. Center for Molecular Medicine and Genetics, School of Medicine, Wayne State University, Detroit, MI

    Henry H. Q. Heng

  2. Department of Biology, York University, Downsview, Ontario, Canada

    Barbara Spyropoulos & Peter B. Moens

Authors
  1. Henry H. Q. Heng
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  2. Barbara Spyropoulos
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  3. Peter B. Moens
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Editor information

Editors and Affiliations

  1. RMIT University Bundoora, Victoria, Australia

    Ian A. Darby

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© 2000 Humana Press Inc.

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Heng, H.H.Q., Spyropoulos, B., Moens, P.B. (2000). DNA-Protein In Situ Covisualization for Chromosome Analysis. In: Darby, I.A. (eds) In Situ Hybridization Protocols. Methods in Molecular Biology™, vol 123. Humana Press. https://doi.org/10.1385/1-59259-677-0:15

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  • DOI: https://doi.org/10.1385/1-59259-677-0:15

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-686-4

  • Online ISBN: 978-1-59259-677-5

  • eBook Packages: Springer Protocols

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