In Situ Hybridization with Nonradioactive Probes

  • Gwen V. Childs
Part of the Methods in Molecular Biology™ book series (MIMB, volume 123)


In situ hybridization takes advantage of the reaction between two complementary single-stranded nucleic acid molecules. These molecules bind by means of hydrogen bonding of complementary base pairs. Hybridization performed in situ includes techniques that detect these hybrids in a cell or tissue section by cytochemistry.


Acetic Anhydride Pituitary Cell cRNA Probe Saline Sodium Citrate Deionized Formamide 
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Gwen V. Childs
    • 1
  1. 1.Department of Anatomy and NeurosciencesUniversity of Texas Medical BranchGalveston

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