Abstract
RNA molecules can fold into extensive structures containing regions of double-stranded duplex, hairpins, internal loops, bulged bases, and pseudo-knotted structures (1). Owing to the complexity of RNA structure, the rules governing sequence-specific RNA-protein recognition are not well understood. RNA-protein interactions are vital for many regulatory processes, especially in gene regulation where proteins specifically interact with binding sites found within RNA transcripts. In the absence of high-resolution crystallographic and nuclear magnetic resonance data, new methods are needed to determine the topology of RNA-protein complexes under physiological conditions. We have devised a new method based on psoralen photochemistry to identify specific contacts in RNA-protein complexes (2,3).
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© 1999 Humana Press Inc.
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Wang, Z., Rana, T.M. (1999). Probing RNA-Protein Interactions by Psoralen Photocrosslinking. In: Haynes, S.R. (eds) RNA-Protein Interaction Protocols. Methods in Molecular Biology™, vol 118. Humana Press. https://doi.org/10.1385/1-59259-676-2:49
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DOI: https://doi.org/10.1385/1-59259-676-2:49
Publisher Name: Humana Press
Print ISBN: 978-0-89603-568-3
Online ISBN: 978-1-59259-676-8
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